Human/Mouse/Rat
Hydroxyacid Oxidase‑1/HAO‑1 Antibody
Human/Mouse/Rat
Hydroxyacid Oxidase‑1/HAO‑1 Antibody Summary
Leu2-Ile370
Accession # Q9UJM8
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human, Mouse, and Rat Hydroxyacid Oxidase‑1/HAO‑1 by Western Blot. Western blot shows lysates of human liver tissue, mouse liver tissue, and rat liver tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human/Mouse/Rat Hydroxyacid Oxidase-1/HAO-1 Monoclonal Antibody (Catalog # MAB6197) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Hydroxyacid Oxidase-1/ HAO-1 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Hydroxyacid Oxidase-1/HAO-1
Glycolate oxidase is a member of the superfamily of the alpha ‑hydroxy acid oxidases (HAO), enzymes that are present in both plants and animals (1). It catalyzes the FMN-mediated oxidation of glycolate to glyoxylate and glyoxylate to oxalate with reduction of oxygen to hydrogen peroxide (2, 3). The co‑factor, FMN, is tightly bound but not covalently linked to the protein. In humans and other vertebrates, HAOs are found primarily in the peroxisomes of liver, kidney, and pancreas. Three HAOs have been identified in humans (4). HAO-1 is most highly expressed in liver and pancreas and is most active on two-carbon substrates such as glycolate. HAO-2 is expressed in liver and kidney and has greater activity against long-chain alpha ‑hydroxy acid substrates such as 2‑hydroxypalmitate. HAO-3 is expressed primarily in the pancreas. HAO contributes to hyperoxaluria, a disorder in which large deposits of calcium oxalate form kidney stones (5).
- Vignaud, C. et al. (2007) Arch. Biochem. Biophys. 465:410.
- Murray, M.S. et al. (2008) Biochemistry, 47:2439.
- Pennati, A. and G. Gadda. (2009) J. Biol. Chem. 284:31214.
- Jones, J.M. et al. (2000) J. Biol. Chem. 275:12590.
- Monico, G.C. et al. (2002) Kidney Int. 62:392.
Product Datasheets
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