Human/Mouse/Rat IGSF4C/SynCAM4 Antibody

Catalog #: MAB41642 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB41642
MAB41642-SP

Detection of Human, Mouse, and Rat IGSF4C/SynCAM4 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat IGSF4C/SynCAM4 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human IGSF4C/SynCAM4 in direct ELISAs. Detects human, mouse, and rat IGSF4C/SynCAM4 in Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 937109

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IGSF4C/SynCAM4
Gln25-Tyr323
Accession # Q8NFZ8

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat IGSF4C/SynCAM4 by Western Blot. Western blot shows lysates of human cerebellum tissue, mouse cerebellum tissue, and rat cerebellum tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat IGSF4C/SynCAM4 Monoclonal Antibody (Catalog # MAB41642) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IGSF4C/SynCAM4 at approximately 55-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGSF4C/SynCAM4

IGSF4C is an immunoglobulin superfamily member that is also known as Nectin-like protein 4 (Necl-4), synaptic cell adhesion molecule 4 (SynCAM4), or tumor suppressor in lung cancer-like 2 (TSLL-2) (1, 2). The four IGSF4 proteins, designated A, B, C and D, are type I transmembrane glycoproteins expressed mainly in neurons, but also in lung, kidney, bladder, prostate and testis (1‑5). Their extracellular domains (ECD) share 35‑50% amino acid (aa) identity and each contain one V‑type Ig-like and two C2-type Ig-like domains. These domains are responsible for Ca⁺⁺-independent homophilic and heterophilic interactions. The 388 aa human IGSF4C contains a 20 aa signal sequence, a 304 aa ECD that shares 98‑99% amino acid identity with mouse, rat, canine and bovine IGSF4C, a 21 aa transmembrane sequence, and a 43 aa cytoplasmic domain. The apparent size of mouse or human IGSF4C may be variably reported as 48‑67 kDa, probably due to differences in glycosylation (2, 5, 8). In the peripheral nervous system, IGSF4C is expressed on Schwann cells, and its internodal interaction with IGSF4A (Necl-1, SynCAM-3) on axons is critical for adhesion and myelination (6‑8). In the brain, all IGSF4 family members are expressed at high levels concurrent with synapse formation (4). In the cerebellum, IGSF4C is expressed on Purkinje cells, with complementary expression of IGSF4 on granule cells (4). Heterophilic interaction with IGSF4D (Necl-3, SynCAM2) has also been identified, but homophilic interaction is unlikely (4, 6). IGSF4C is also proposed as a tumor suppressor that is downregulated in many prostate cancers and gliomas (1, 5).

Long Name

Immunoglobulin Superfamily, Member 4B

Entrez Gene IDs

199731 (Human); 260299 (Mouse)

Alternate Names

CADM4; cell adhesion molecule 4; IGSF4C; IGSF4Cnectin-like 4; Immunoglobulin superfamily member 4C; immunoglobulin superfamily, member 4C; Necl-4; NECL4synCAM4; Nectin-like protein 4; SynCAM4; TSLC1-like 2; TSLC1-like protein 2; TSLL2; TSLL2SynCAM4