Human/Mouse/Rat IMP2 Antibody

Catalog #: AF5305 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5305
AF5305-SP

Detection of Human/Mouse/Rat IMP2 by Western Blot.

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Human/Mouse/Rat IMP2 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects endogenous human, mouse and rat IMP2 isoforms in Western blots. Reactivity with other IMP family members is unknown.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human IMP2
Met1-Thr220
Accession # Q9Y6M1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse/Rat IMP2 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, A431 human epithelial carcinoma cell line, Caco-2 human colorectal adenocarcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and Y3-Ag rat myeloid cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat IMP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5305) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for IMP2 at approximately 62 to 68 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IMP2

IMP2 (IGF-II mRNA-binding protein 2; also VICKZ family member 2) is a 62 kDa member of the RRM IMP/VICKZ family of proteins. It is expressed in oocytes, spermatogonia, Leydig cells and follicular granulosa cells. IMP2 binds to AUF1, a binding protein for the AU-rich motifs of mRNA, and facilitates the degradation of cytokine and protooncogene mRNAs. Human IMP2 is 556 amino acids (aa) in length. It contains two RNA recognition regions (aa 3‑76 and 82‑157), and four KH domains (aa 193‑532) that mediate RNA binding. There are multiple splice variants. One shows an insertion of 43 aa after Asn357, a second shows a 17 aa substitution for the N-terminal 80 aa, a third shows a combination of the prior two, and a fourth shows a 15 aa substitution for aa 414‑556. Over aa 1‑220, human IMP2 is 89% aa identical to mouse IMP2.

Long Name

Insulin-like Growth Factor 2 mRNA Binding Protein 2

Entrez Gene IDs

10644 (Human); 319765 (Mouse); 303824 (Rat)

Alternate Names

Hepatocellular carcinoma autoantigen p62; IF2B2; IGF2BP2; IGF-II mRNA-binding protein 2; IMP2; IMP-2IGF2 mRNA-binding protein 2; IMP2VICKZ family member 2; insulin-like growth factor 2 mRNA binding protein 2; insulin-like growth factor 2 mRNA-binding protein 2; p62; VICKZ2