Human/Mouse/Rat IMPA1/IMP1 Antibody

Catalog #: MAB98901 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB98901-100
MAB98901-SP

Detection of Human. Mouse, and Rat IMPA1/IMP1 by Western Blot.

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All IMPA1/IMP1 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat IMPA1/IMP1 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human IMPA/IMPA1 Peptide in direct ELISAs. Detects human, mouse, and rat IMPA/IMPA1 in Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 984603

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Synthetic peptide containing human IMPA/IMPA1 Peptide
Accession # P29218

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human. Mouse, and Rat IMPA1/IMP1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, NIH-3T3 mouse embryonic fibroblast cell line, and C6 rat glioma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat IMPA1/IMP1 Monoclonal Antibody (Catalog # MAB98901) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IMPA1/IMP1 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IMPA1/IMP1

IMPA-1/inositol(myo)-1(or 4)-monophosphatase 1 is a ubiquitous enzyme with broad specificity, which is responsible for the provision of inositol required for synthesis of phosphatidylinositol and polyphosphoinositides. IMPA-1 dephosphorylates myo-inositol monophosphatate to modulate intracellular signal transduction. IMPA-1 forms homodimers, and is abundantly expressed in brain. The magnesium-dependent phosphatase activity is inhibited by lithium, thereby depressing myo-inositol production, which may explain the anti-depressive and anti-manic effects of lithium therapy in bipolar disorder.

Long Name

Inositol Monophosphatase 1

Entrez Gene IDs

3612 (Human); 55980 (Mouse); 83523 (Rat)

Alternate Names

EC 3.1.3.25; IMP 1; IMP; IMP1; IMPA; IMPA1; IMPase 1; inositol monophosphatase 1; inositol(myo)-1(or 4)-monophosphatase 1; Inositol-1(or 4)-monophosphatase 1; Lithium-sensitive myo-inositol monophosphatase A1; MRT59; Myo-Inositol Monophosphatase 1