Home / Inorganic Pyrophosphatase/PPA1 / Human/Mouse/Rat Inorganic Pyrophosphatase/PPA1 Antibody

Human/Mouse/Rat Inorganic Pyrophosphatase/PPA1 Antibody

Catalog #: MAB6557
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB6557
MAB6557-SP
Detection of Human, Mouse, and Rat Inorganic Pyrophosphatase/PPA1 by Western Blot.
1 Image
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Inorganic Pyrophosphatase/PPA1 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human Inorganic Pyrophosphatase/PPA1 in direct ELISAs and human, mouse, and rat Inorganic Pyrophosphatase/PPA1 in Western blots.
Source
Monoclonal Mouse IgG2B Clone # 840941
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Inorganic Pyrophosphatase/PPA1
Met1-Asn289
Accession # Q15181
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat Inorganic Pyrophosphatase/PPA1 antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat Inorganic Pyrophosphatase/PPA1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Neuro-2A mouse neuroblastoma cell line, and C6 rat glioma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Inorganic Pyrophosphatase/PPA1 Monoclonal Antibody (Catalog # MAB6557) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Inorganic Pyrophosphatase/PPA1 at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Inorganic Pyrophosphatase/PPA1

PPA1 (Inorganic pyrophosphatase 1; also PPase and IOPPP) is a 32-36 kDa cytoplasmic member of the PPase family of enzymes. It is ubiquitously expressed, and acts on di- (or pyro) phosphate, generating orthophosphate in a Mg+-dependent manner. This activity can both generate energy for cells, or in the case of osteoblasts, provide raw material for calcification. The consumption of pyrophosphate may also remove inhibitors of enzymes such as guanylyl cyclase, and PPA1 itself is also reported to stimulate gene expression. Human PPA1 is 289 amino acids (aa) in length. There is one pyrophosphatase domain between aa 42-255, and two utilized acetylation sites at Lys 57 and Lys228. PPA1 is known to form homodimers. There is one potential alternative start site at Met46. Full-length human PPA1 shares 94% aa sequence identity with mouse PPA1.

Entrez Gene IDs
5464 (Human); 852296 (Yeast)
Alternate Names
diphosphate phosphohydrolase; EC 3.6.1.1; inorganic diphosphatase; inorganic pyrophosphatase 1; inorganic pyrophosphatase; IOPPP; IOPPPMGC111556; IPP1; PP1; PPA1; PPase; PPcytosolic inorganic pyrophosphatase; pyrophosphatase (inorganic) 1; pyrophosphatase (inorganic); pyrophosphatase 1; Pyrophosphate phospho-hydrolase; SID6-8061

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citation for Human/Mouse/Rat Inorganic Pyrophosphatase/PPA1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Understanding Protein-Nanoparticle Interaction: A New Gateway to Disease Therapeutics.
    Authors: Giri K, Shameer K, Zimmermann MT et al.
    Bioconjug. Chem.

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human/Mouse/Rat Inorganic Pyrophosphatase/PPA1 Antibody

There are currently no reviews for this product. Be the first to review Human/Mouse/Rat Inorganic Pyrophosphatase/PPA1 Antibody and earn rewards!

Have you used Human/Mouse/Rat Inorganic Pyrophosphatase/PPA1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review