Human/Mouse/Rat Kynurenine 3-Monooxygenase/KMO Antibody

Catalog #: MAB8050
3 Citations Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB8050-100
MAB8050-SP

Detection of Human, Mouse, and Rat Kynurenine 3-Monooxygenase/KMO by Western Blot.

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All Kynurenine 3-Monooxygenase/KMO Antibodies

Product Details

Citations (3)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Kynurenine 3-Monooxygenase/KMO Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human Kynurenine 3-Monooxygenase/KMO in direct ELISAs. Detects human, mouse, and rat Kynurenine 3-Monooxygenase/KMO in Western blot.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2493A

Purification

Protein A or G purified from cell culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Kynurenine 3-Monooxygenase/KMO
Asp2-Leu441
Accession # O15229

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

3-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat Kynurenine 3-Monooxygenase/KMO by Western Blot. Western blot shows lysates of mouse liver tissue, rat spleen tissue, and MDA-MB-453 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Mouse/Rat Kynurenine 3-Monooxygenase/KMO Monoclonal Antibody (Catalog # MAB8050) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Kynurenine 3-Monooxygenase/KMO at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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Kynurenine 3-Monooxygenase/KMO in Human Placenta. Kynurenine 3-Monooxygenase/KMO was detected in immersion fixed paraffin-embedded sections of human placenta using Rabbit Anti-Human/Mouse/Rat Kynurenine 3-Monooxygenase/KMO Monoclonal Antibody (Catalog # MAB8050) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in syncytiotrophoblast cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kynurenine 3-Monooxygenase/KMO

Kynurenine 3-Monooxygenase (KMO), also known as Kynurenine 3-Hydroxylase, is a part of the kynurenine pathway of tryptophan degradation (1). KMO catalyzes the NADPH- and flavin adenine dinucleotide (FAD)-dependent 3-hydroxylation of kynurenine to 3-hydroxykynurenine (3-HK). 3-HK is neurotoxic via the generation of hydrogen peroxide (2) and through the excitotoxic effects of its downstream metabolite quinolinic acid (3). The levels of 3-HK and quinolinic acid are increased in the brain with Alzheimer's disease and Huntington's disease (1). Inhibition of KMO was shown to reverse cognitive and motor deficits in mouse models of those diseases via an increase in neuroprotective kynurenic acid (4). KMO is found in the mitochondrial outer membrane of microglial cells in the brain and dendritic cells and macrophages in the periphery (1).

References

Schwarcz, R. et al. (2012) Nat. Rev. Neurosci. 13:465.
Okuda, S. et al. (1996) Proc. Natl. Acad. Sci. 93:12553.
Stone, T.W. and M.N. Perkins. (1981) Eur. J. Pharmacol. 72:411.
Zwilling, D. et al. (2011) Cell 145:863.

Entrez Gene IDs

8564 (Human); 98256 (Mouse); 59113 (Rat)

Alternate Names

KMO; kynurenine 3-monooxygenase (kynurenine 3-hydroxylase); Kynurenine 3Monooxygenase; Kynurenine 3-Monooxygenase

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse/Rat Kynurenine 3-Monooxygenase/KMO Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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