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Human/Mouse/Rat LAR Antibody

Catalog #: AF3004 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3004
AF3004-SP
Detection of Human/Mouse/Rat LAR by Western Blot.
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat LAR Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat LAR in Western blots. In Western blots, this antibody detects both full-length protein and the cleaved LAR extracellular (E) domain. The antibody does not cross react with PTPR sigma.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human LAR
Ala27-Glu1251
Accession # NP_569707
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/Rat LAR antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat LAR by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Neuro-2A mouse neuroblastoma cell line, and Y3-Ag rat myeloid cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat LAR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3004) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Bands were detected for LAR at approximately 207 kDa for full-length LAR and 139 kDa for cleaved LAR (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LAR

Leucocyte Antigen-Related (LAR) tyrosine phosphatase, also known as Protein Tyrosine Phoshatase, Receptor-type F (PTPRF), is an integral membrane protein with a non-glycosylated molecular weight of 207 kDa. The extracellular domain is cleaved near the cell membrane by a subtilisin-like endoprotease to a molecular weight of 139 kDa. Depending on cellular conditions, the extracellular domain may remain associated with the rest of the molecule or can be shed into the extracellular medium. This antibody was made against the extracellular domain of LAR and detects both full-length and cleaved LAR.

Long Name
Leukocyte Antigen-Related Tyrosine Phosphatase
Entrez Gene IDs
5792 (Human); 104121 (Mouse)
Alternate Names
LAR; LCA-homolog; protein tyrosine phosphatase, receptor type, F; PTPRF; receptor type, F polypeptide; receptor-linked protein-tyrosine phosphatase LAR; receptor-type tyrosine-protein phosphatase F

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Reviews for Human/Mouse/Rat LAR Antibody

Average Rating: 5 (Based on 1 Review)

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Human/Mouse/Rat LAR Antibody
By Sijia He on 11/01/2018
Application: WB Sample Tested: mouse primary hepatocyte Species: Mouse

Tried this antibody, works very well ,the bands were specific and clean, located between 115-250 kDa area. Highly recommend.

Western Blot Human/Mouse/Rat LAR Antibody AF3004