Human/Mouse/Rat LMW-PTP/ACP1 Antibody

Catalog #: MAB5075 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5075
MAB5075-SP

Detection of Human LMW-PTP/ACP1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat LMW-PTP/ACP1 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat LMW-PTP/ACP1 in Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 475417

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human LMW-PTP/ACP1
Ala2-His158
Accession # P24666

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human LMW-PTP/ACP1 by Western Blot. Western blot shows lysates of CHP-100 human neuroblastoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat LMW-PTP/ACP1 Monoclonal Antibody (Catalog # MAB5075) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for LMW-PTP/ACP1 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LMW-PTP/ACP1

Low molecular weight protein tyrosine phosphatase (LMW-PTP), also known as Acid Phosphatase 1 (ACP1), is an 18 kDa cytosolic phosphatase that is unrelated to other tyrosine phosphatases, such as PTP1B. Tyrosine phosphorylation of LMW-PTP increases its activity 20-fold and affects its ability to dephosphorylate targets such as the PDGF receptor. Cancer cells overexpressing LMW-PTP have a higher proliferative rate and generate tumors that are larger than untransfected controls. Colon adenocarcinomas induced by dimethylhydrazine also have elevated levels of LMW-PTP, suggesting that this phosphatase may be a marker for oncogenic transformation. Human LMW-PTP shares 86% and 87% amino acid sequence identity with mouse and rat LMW-PTP, respectively.

Long Name

Low Molecular Weight Phosphotyrosine Protein Phosphotase

Entrez Gene IDs

52 (Human); 11431 (Mouse); 24161 (Rat)

Alternate Names

acid phosphatase 1, soluble; acid phosphatase of erythrocyte; ACP1; Adipocyte acid phosphatase; cytoplasmic phosphotyrosyl protein phosphatase; EC 3.1.3.2; EC 3.1.3.48; HAAP; LMWPTP; LMW-PTP; LMW-PTPase; Low molecular weight cytosolic acid phosphatase; low molecular weight phosphotyrosine protein phosphatase; MGC111030; MGC3499; protein tyrosine phosphatase; Red cell acid phosphatase 1