Human/Mouse/Rat LMW-PTP/ACP1 Antibody

Catalog #: AF5075 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5075
AF5075-SP

Detection of Human, Mouse, and Rat LMW‑PTP/ACP1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat LMW-PTP/ACP1 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat LMW‑PTP/ACP1 in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human LMW‑PTP/ACP1
Ala2-His158
Accession # P24666

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat LMW‑PTP/ACP1 by Western Blot. Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, human placenta tissue, TF-1 human erythroleukemic cell line, C2C12 mouse myoblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat LMW-PTP/ACP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5075) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LMW-PTP/ACP1 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LMW-PTP/ACP1

LMW-PTP (Low Molecular Weight Protein Tyrosine Phosphatase; also Red cell acid phosphatase 1/ACP1, HCPTP, and HAAP/Adipocyte acid phosphatase) is a 17‑18 kDa member of the LMWPTP family, PTP superfamily of enzymes. It is widely expressed, being found in cells diverse as B and T cells, endothelial cells and vascular smooth muscle cells. LMW-PTP dephosphorylates multiple substrates, often in a cell-dependent manner. Among the substrates for LMW-PTP include PDGR beta, EphA2 and beta -catenin. PMW-PTP is found intracellularly, generally associated with two cytoplasmic pools, one in the cytosol and the other accompaning the cytoskeleton. Human LMW-PTP is 158 amino acids (aa) in length. The catalytic region spans aa 9-154, and there are two acetylation sites at Ala2 and Lys156. Phosphorylation on Tyr132 and Tyr133 occurs on cytoskeleton-associated LMW-PTP, and has the effect of increasing its phosphatase activity. There are three alternative splice variants reported for LMW-PTP. One shows a 34 aa substitution for aa 41-74, a second shows a deletion of aa 41-74, while a third contains a 35 aa substitution for aa 78-158. The last two isoforms may function a negative regulators of LMW-PTP activity. Full-length LMW-PTP shares 86% aa sequence identity with mouse LMW-PTP.

Long Name

Low Molecular Weight Phosphotyrosine Protein Phosphotase

Entrez Gene IDs

52 (Human); 11431 (Mouse); 24161 (Rat)

Alternate Names

acid phosphatase 1, soluble; acid phosphatase of erythrocyte; ACP1; Adipocyte acid phosphatase; cytoplasmic phosphotyrosyl protein phosphatase; EC 3.1.3.2; EC 3.1.3.48; HAAP; LMWPTP; LMW-PTP; LMW-PTPase; Low molecular weight cytosolic acid phosphatase; low molecular weight phosphotyrosine protein phosphatase; MGC111030; MGC3499; protein tyrosine phosphatase; Red cell acid phosphatase 1