Human/Mouse/Rat MEK2 Antibody

Name: Human/Mouse/Rat MEK2 Antibody
Brand: R&D Systems
SKU: MAB2855
Rating: 5 (1 reviews)

Catalog #: MAB2855 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2855
MAB2855-SP

Detection of Human/Mouse/Rat MEK2 by Western Blot.

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Human/Mouse/Rat MEK2 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat MEK2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) MEK1 or rhMEK5 is observed.

Source

Monoclonal Mouse IgG_2A Clone # 300317

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human MEK2
Met1-Val400
Accession # P36507

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse/Rat MEK2 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, U937 human histiocytic lymphoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and NRK rat normal kidney cell. PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human/Mouse/Rat MEK2 Monoclonal Antibody (Catalog # MAB2855) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human MEK1 and Recombinant Human Active MEK2 (Catalog # 2855-KS) (5 ng/lane) were included. A specific band for MEK2 was detected at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Immunocytochemistry

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MEK2 in HeLa Human Cell Line. MEK2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat MEK2 Monoclonal Antibody (Catalog # MAB2855) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MEK2

Mitogen-activated protein kinase kinase 2 (MEK2 or MAP2K2), also known as MKK2,belongs to the STE family of kinases. Both MEK2 and the related MEK1 aredual‑specificity kinases, phosphorylating and activating the mitogen-activated proteinkinases ERK1 and ERK2 at T and Y positions within the phosphoacceptor sequence T-E-Y. Activation of MEK2 by Raf occurs through phosphorylation at S222 and S226.

Long Name

Mitogen-activated Protein Kinase Kinase 2

Entrez Gene IDs

5605 (Human); 26396 (Mouse)

Alternate Names

ERK activator kinase 2; MAP2K2; MAPKK 2; MEK 2; MEK2; MEK2EC 2.7.12.2; mitogen-activated protein kinase kinase 2; MKK2; MKK2FLJ26075; p45; PRKMK2MAPKK2