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Human/Mouse/Rat MIOS Antibody

Catalog #: MAB9289
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB9289-100
MAB9289-SP

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Detection of Human MIOS by Western Blot.
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Citations (1)
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Human/Mouse/Rat MIOS Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human MIOS in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 955413
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human MIOS synthetic peptide
Accession # Q9NXC5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human MIOS antibody by Western Blot. View Larger

Detection of Human MIOS by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, Jurkat human acute T cell leukemia cell line, 3T3-L1 mouse embryonic fibroblast adipose-like cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat MIOS Monoclonal Antibody (Catalog # MAB9289) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MIOS at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MIOS

MIOS is a member of the octameric GATOR (GTPase-activating protein (GAP) activity toward RAGs) complex.  MIOS and fellow GATOR2 subcomplex members WDR24, WDR59, SEH1L and SEC13 inhibit GATOR1 subcomplex members DEPDC5, NPRL2 and NPRL3.  This inhibition by GATOR2 positively regulates mTORC1 signaling, an activity modulated by energy levels, growth factors, and amino acids.  Human MIOS is 875 amino acids (aa) in length, and contains six N-terminal WD40 repeats and a C-terminal zinc finger-like domain.  Over aa 620-875, human MIOS shares 98% identity with mouse and rat MIOS.

Long Name
Missing Oocyte, Meiosis Regulator
Entrez Gene IDs
54468 (Human); 252875 (Mouse); 362324 (Rat)
Alternate Names
FLJ20323; MIO; MIOS; missing oocyte, meiosis regulator, homolog (Drosophila); WD repeat-containing protein mio

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Citation for Human/Mouse/Rat MIOS Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Seh1 targets GATOR2 and Nup153 to mitotic chromosomes
    Authors: Melpomeni Platani, Itaru Samejima, Kumiko Samejima, Masato T. Kanemaki, William C. Earnshaw
    Journal of Cell Science

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