Human/Mouse/Rat MKK6 Antibody

Catalog #: AF16041 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF16041
AF16041-SP

Detection of Human/Mouse/Rat MKK6 by Western Blot.

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Human/Mouse/Rat MKK6 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat MKK6.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human MKK6
Ser2-Asp334
Accession # P52564

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

See below

Knockout Validated

MKK6 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in MKK6 knockout HeLa cell line.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse/Rat MKK6 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, C6 rat glioma cell line, and B16-F1 mouse melanoma cell line. PVDF membrane was probed with 0.1 µg/mL Goat Anti-Human/Mouse/Rat MKK6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF16041) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human MKK6 (2 ng) was included. A specific band for MKK6 was detected at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Knockout Validated

Western Blot Shows Human MKK6 Antibody Specificity by Using Knockout Cell Line.

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Western Blot Shows Human MKK6 Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and MKK6 knockout HeLa cell line (KO). PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human/Mouse/Rat MKK6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF16041) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MKK6 at approximately 39 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MKK6

Mitogen-activated protein kinase kinase 6 (MKK6), also known as MEK6, and the closely related MKK3 are activated by pro-inflammatory cytokines and environmental stresses (1, 2). Both MKK3 and MKK6 are dual specificity protein kinases, phosphorylating and activating the p38 MAP kinases at Thr and Tyr positions within the phosphoacceptor sequence Thr-Gly-Tyr (3). Activation of MKK6 occurs through phosphorylation at Ser207 and Thr211 (4) by several upstream MAPK kinase kinases, including ASK1 and TAK1 (5, 6). Purification of E. coli-expressed wild-type active MKK6 capable of p38 alpha phosphorylation has been previously noted (7).

References

Goedert, M. et al. (1997) EMBO J. 16:3563.
Moriguchi, T. et al. (1996) J. Biol. Chem. 271:26981.
Raingeaud, J. et al. (1995) J. Biol. Chem. 270:7420.
Han, J. et al. (1996) J. Biol. Chem. 271:2886.
Ichijo, H. et al. (1997) Science 275:90.
Moriguchi, T. et al. (1996) J. Biol. Chem. 271:13675.
Keesler, G. et al. (1998) Protein Expr. Purif. 14:221.

Long Name

Mitogen-activated Protein Kinase Kinase 6

Entrez Gene IDs

5608 (Human); 26399 (Mouse); 114495 (Rat)

Alternate Names

MAP kinase kinase 6; MAP2K6; MAPK/ERK kinase 6; MAPKK6dual specificity mitogen-activated protein kinase kinase 6; MEK6; MEK6MAPKK 6; mitogen-activated protein kinase kinase 6; MKK6; MKK6MEK 6; PRKMK6; PRKMK6protein kinase, mitogen-activated, kinase 6 (MAP kinase kinase 6); SAPKK3; SAPKK3EC 2.7.12.2

Product Datasheets

Product Datasheet

COA

SDS

Product Specific Notices

The purchase of this product conveys to the buyer the limited, non-exclusive, non-transferable right (without the right to resell, repackage, or further sublicense) to use these reagents for research purposes only. No other license is granted to the buyer whether expressly, by implication, by estoppel or otherwise. In particular, the purchase of this product does not include nor carry any right or license to use, develop, or otherwise exploit this product commercially, which includes without limitation, provision of services to a third party, generation of commercial databases, clinical diagnostics or therapeutics, or drug development. This product is sold pursuant to a license from the University of Massachusetts, and the University of Massachusetts reserves all other rights to use these reagents. For information on purchasing a license for uses other than research, please contact the University of Massachusetts, Office of Technology Management, 333 South Street, Suite 400, Shrewsbury, Massachusetts, 01545.