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Human/Mouse/Rat N-WASP Antibody

Catalog #: AF3854
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3854
AF3854-SP
Detection of Human, Mouse, and Rat N-WASP by Western Blot.
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Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat N-WASP Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat N-WASP in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human N-WASP
Leu21-Asn270
Accession # O00401
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
Immersion fixed A431 human epithelial carcinoma cell line treated with recombinant human EGF (Catalog # 236-EG-01M)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat N-WASP antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat N-WASP by Western Blot. Western blot shows lysates of DU145 human prostate carcinoma cell line, Neuro-2A mouse neuroblastoma cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat N-WASP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3854) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for N-WASP at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry N-WASP antibody in Rat Brain Tissue by Immunohistochemistry (IHC-Fr). View Larger

N‑WASP in Rat Brain Tissue. N-WASP was detected in perfusion fixed frozen sections of rat brain (cortex) tissue using Goat Anti-Human/Mouse/Rat N-WASP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3854) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to neuronal cell cytoplasm. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: N-WASP

N-WASP is a ubiquitously expressed member of the WASP family of proteins. The 65 kDa, 505 amino acid, N-WASP seems to play a central role as a node interconnecting various actin networks. Over the region used as immunogen, human N-WASP is greater than 98% identical to the corresponding mouse and canine protein sequences.

Long Name
Neural Wiskott-Aldrich Syndrome Protein
Entrez Gene IDs
8976 (Human); 73178 (Mouse); 296936 (Rat)
Alternate Names
NWASP; N-WASP; N-WASPneural WiskNWASP; TRS4; WASL; WiskDKFZp779G0847; WiskMGC48327

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Citation for Human/Mouse/Rat N-WASP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Targeting Src family kinase member Fyn by Saracatinib attenuated liver fibrosis in vitro and in vivo
    Authors: G Du, J Wang, T Zhang, Q Ding, X Jia, X Zhao, J Dong, X Yang, S Lu, C Zhang, Z Liu, Z Zeng, R Safadi, R Qi, X Zhao, Z Hong, Y Lu
    Cell Death Dis, 2020-02-12;11(2):118.
    Species: Human
    Sample Types: Cell Culture Lysates
    Applications: Western Blot

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