Human/Mouse/Rat NADK Antibody

Catalog #: AF8009 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF8009
AF8009-SP

Detection of Human, Mouse, and Rat NADK by Western Blot.

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Human/Mouse/Rat NADK Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse and rat in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human NADK
Met1-Gly446
Accession # O95544

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat NADK by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human/Mouse/Rat NADK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8009) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for NADK at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NADK

NADK (Nicotinamide Adenine Dinucleotide Kinase; also Poly(P)/ATP NAD kinase) is a 49-52 kDa cytosolic member of the NAD kinase family of enzymes. Although it is widely expressed, it is not found in skeletal muscle. NADK forms homotetramers and catalyzes the generation of NADP from NAD through the hydrolysis of ATP. NADP plays a role in fatty acid synthesis plus steroidogenesis, and provides a substrate for the generation of NADPH that serves to regulate the expression of reductases and antioxidant proteins. Human NADK is 446 amino acids (aa) in length. It contains one kinase domain (aa 105-429) plus a C-terminal Ala- and Glu-rich segment. There are four utilized phosphorylation sites at Ser46, Ser48, Ser50 and Ser64. The potential exists for an alternate start site at Met134, and two isoform variants are reported, one that contain a 56 aa substitution for aa 1-88, and another that possesses a 105 aa substitution for Met88 coupled to a 41 aa insertion after Glu131. Full-length NADK (aa 1-446) shares 91% aa sequence identity with mouse NADK. Recently, a human mitochondrial NADK was identified (termed C5orf33), but found to share only 19% aa sequence identity with cytosolic NADK.

Long Name

NAD Kinase

Entrez Gene IDs

65220 (Human); 192185 (Mouse)

Alternate Names

dJ283E3.1; DKFZp686L22239; EC 2.7.1; EC 2.7.1.23; FLJ13052; FLJ37724; FLJ54695; FLJ77769; FLJ78247; FLJ78307; MGC1900; NAD kinase; NADK; Poly(P)/ATP NAD kinase