Human/Mouse/Rat Neurofascin Antibody Summary
Ile25-Ala1031
Accession # NP_446361
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of Human, Mouse, and Rat Neurofascin by Western Blot. Western blot shows lysates of rat brain tissue, mouse brain tissue, human brain (cortex) tissue, human brain (cerebellum) tissue, and human brain (hippocampus) tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat Neurofascin Monoclonal Antibody (Catalog # MAB3235) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for Neurofascin at approximately 140 to 155 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Neurofascin
Neurofascin 155 (NF155) is a type I transmembrane glycoprotein that belongs to the L1CAM family of cell adhesion proteins (1, 2). The rat NF155 cDNA encodes a 1240 amino acid (aa) precursor that contains a 24 aa signal sequence, a 1086 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 109 aa cytoplasmic domain. The ECD consists of six Ig-like domains and four fibronectin type III repeats, the second of which has an RGD motif. A splice variant of Neurofascin, NF186, lacks the RGD-containing fibronectin type III domain but instead has a mucin-like domain and an additional non-RGD fibronectin type III domain (3). Within shared regions of the ECD, rat NF155 shares 45% and 39% aa sequence identity with rat Nr-CAM and L1CAM, respectively, and 98% aa sequence identity with human and mouse NF155. NF155 is transiently expressed by oligodendrocytes at the onset of axon myelination, whereas NF186 is neuronally expressed in nodes of Ranvier (4‑6). Clustering of NF155 in paranodal oligodengroglia lipid raft domains is stabilized by dimerization of its cytoplasmic domains and association with intracellular ankyrin (6‑9). NF155 interacts with axonal contactin and plays a role in node of Ranvier formation and the establishment of saltatory conduction (5, 9‑12). The ECD of NF155 is cleaved from oligodengroglia membranes by metalloproteases, a process which is required for NF155 transport from the glial cell body to the axoglial junction (13). In addition to distinct expression patterns, Neurofascin isoforms have different functional properties. NF155 promotes neuronal adhesion and neurite outgrowth, whereas NF186 inhibits neuronal adhesion (4, 7, 13).
- Sherman, D.L. and P.J. Brophy (2005) Nat. Rev. Neurosci. 6:683.
- Coman, I. et al. (2005) J. Neurol. Sci. 233:67.
- Volkmer, H. et al. (1992) J. Cell Biol. 118:149.
- Koticha, D. et al. (2005) Mol. Cell. Neurosci. 30:137.
- Collinson, J.M. et al. (1998) Glia 23:11.
- Schafer, D.P. et al. (2004) J. Neurosci. 24:3176.
- Maier, O. et al. (2005) Mol. Cell. Neurosci. 28:390.
- Zhang, X. et al. (1998) J. Biol. Chem. 273:30785.
- Sherman, D.L. et al. (2005) Neuron 48:737.
- Gollan, L. et al. (2003) J. Cell Biol. 163:1213.
- Charles, P. et al. (2002) Curr. Biol. 12:217.
- Volkmer, H. et al. (1996) J. Cell Biol. 135:1059.
- Maier, O. et al. (2006) Exp. Cell Res. 312:500.
Product Datasheets
Citations for Human/Mouse/Rat Neurofascin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
3
Citations: Showing 1 - 3
Filter your results:
Filter by:
-
The incubation of cocaine craving is dissociated from changes in glial cell markers within prefrontal cortex and nucleus accumbens of rats
Authors: Webb SM, Sacramento AD, McCloskey MA Et al.
Addict Neurosci
-
An iPSC model of hereditary sensory neuropathy-1 reveals L-serine-responsive deficits in neuronal ganglioside composition and axoglial interactions
Authors: Alex J. Clark, Umaiyal Kugathasan, Georgios Baskozos, David A. Priestman, Nadine Fugger, Museer A. Lone et al.
Cell Reports Medicine
-
IgG4 anti-neurofascin155 antibodies in chronic inflammatory demyelinating polyradiculoneuropathy: Clinical significance and diagnostic utility of a conventional assay
Authors: Katsunori Ikewaki
J. Neuroimmunol., 2016-11-09;0(0):.
Species: Human
Sample Types: Serum
Applications: Western Blot
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human/Mouse/Rat Neurofascin Antibody
There are currently no reviews for this product. Be the first to review Human/Mouse/Rat Neurofascin Antibody and earn rewards!
Have you used Human/Mouse/Rat Neurofascin Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
