Human/Mouse/Rat NIPP1 Antibody

Catalog #: AF7240 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7240
AF7240-SP

Detection of Human, Mouse, and Rat NIPP1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat NIPP1 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat NIPP1 in Western blots and detects recombinant human NIPP1 in direct ELISAs.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human NIPP1
Ala2-Ile351
Accession # Q12972

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat NIPP1 by Western Blot. Western blot shows lysates of Saos-2 human osteosarcoma cell line, Jurkat human acute T cell leukemia cell line, M1 mouse myeloid leukemia cell line, NRK rat normal kidney cell line, and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse/Rat NIPP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7240) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for NIPP1 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NIPP1

NIPP1 (Nuclear Inhibitory polypeptide of Protein Phosphatase 1; also ARD-1 and PPP1R8) is a 41 kDa intracellular protein that belongs to no known molecular family. Although it is ubiquitously expressed, it does undergo alternative splicing, and distinct isoforms are associated with distinct tissues. In the nucleus, NIPP1 complexes with PP1c, targeting it to either RNA or chromatin. This is accompanied by the additional recruitment of other molecules such as Sap155 and PRC2. When nonphosphorylated, NIPP1 blocks PP1c activity; when phosphorylated, NIPP1 allows for PP1c activity, and this impacts the processes of both pre-mRNA splicing and gene silencing. Human NIPP1 is 351 amino acids (aa) in length. It contains an N-terminal FHA domain (aa 49-101), an NLS (aa 185-209), a PP1 binding site (aa 191-210), another NLS (aa 210-240) and an RNA binding site (aa 330-351). There are two potential splice variants. Both possess alternative start sites, one at Met143 (called NIPP1 beta ), and another at Met225 (called ARD-1 or NIPP1 gamma ). Full-length human NIPP1 shares 98% aa sequence identity with mouse NIPP1.

Long Name

Nuclear Inhibitor of Protein Phosphatase 1

Entrez Gene IDs

5511 (Human); 100336 (Mouse); 313030 (Rat)

Alternate Names

activator of RNA decay; ARD1; ard-1; ARD1nuclear inhibitor of protein phosphatase 1; NIPP1; NIPP1nuclear inhibitor of protein phosphatase-1; NIPP-1nuclear subunit of PP-1; PPP1R8; PRO2047; Protein phosphatase 1 regulatory inhibitor subunit 8; protein phosphatase 1 regulatory subunit 8; protein phosphatase 1, regulatory (inhibitor) subunit 8; RNase E