Home / Nrf1 / Human/Mouse/Rat Nrf1 Antibody

Human/Mouse/Rat Nrf1 Antibody

Catalog #: MAB5306
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB5306
MAB5306-SP
Detection of Human, Mouse, and Rat Nrf1 by Western Blot.
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Product Details
Citations (2)
FAQs
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Nrf1 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects endogenous human, mouse and rat Nrf1 at 85 kDa in Western blots.
Source
Monoclonal Mouse IgG2A Clone # 492413
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Nrf1
Met1-Lys742
Accession # AAH10623
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat Nrf1 antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat Nrf1 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, MOLT-4 human acute lymphoblastic leukemia cell line, SH-SY5Y human neuroblastoma cell line, A20 mouse B cell lymphoma cell line, and Y3-Ag rat myeloid cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat Nrf1 Monoclonal Antibody (Catalog # MAB5306) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Nrf1 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Western Blot Detection of Rat Nrf1 by Western Blot View Larger

Detection of Rat Nrf1 by Western Blot Effect of myricetin on mitochondrial biogenesis regulators under acute hypoxia detecting by western blotting.(A) Representative images of western blots for genes related to mitochondrial biogenesis in skeletal muscle tissue of rats under acute hypoxia exposure; (B) Representative images of western blots for genes related to mitochondrial biogenesis in L6 muscle cell line. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0124727), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Rat Nrf1 by Western Blot View Larger

Detection of Rat Nrf1 by Western Blot Effect of myricetin on mitochondrial biogenesis regulators under acute hypoxia detecting by western blotting.(A) Representative images of western blots for genes related to mitochondrial biogenesis in skeletal muscle tissue of rats under acute hypoxia exposure; (B) Representative images of western blots for genes related to mitochondrial biogenesis in L6 muscle cell line. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0124727), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nrf1

NF-E2-related factor 1 (Nrf1; also transcription factor 11 and LCR-F1) is a ubiquitously expressed transcription factor and member of the bZIP family and the CNC subfamily. Human Nrf1 is 772 amino acids (aa) in length. Like other members of the bZIP family, Nrf1 contains heptad repeats of leucine and hydrophobic residues within a putative amphipathic helical domain of 40 aa and is preceded by a 30 aa domain rich in arginine and lysine residues. A splicing variant produces two isoforms for Nrf1. Isoform 2 has a 30 aa deletion corresponding to aa 242‑271 in isoform 1. Human Nrf1 is 96% and 93% identical to bovine and mouse Nrf1, respectively.

Long Name
NF-E2-related Factor 1
Entrez Gene IDs
4779 (Human); 18023 (Mouse); 360610 (Rat)
Alternate Names
Alpha palindromic-binding protein; ALPHA-PAL; EWG; HBZ17; LCR-F1; NFE2L1; Nrf1; NRF-1; nuclear respiratory factor 1; TCF11

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Citations for Human/Mouse/Rat Nrf1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. GATA3 induces mitochondrial biogenesis in primary human CD4+ T cells during DNA damage
    Authors: LA Callender, J Schroth, EC Carroll, C Garrod-Ket, LEL Romano, E Hendy, A Kelly, P Lavender, AN Akbar, JP Chapple, SM Henson
    Nature Communications, 2021-06-07;12(1):3379.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Protective effects of myricetin on acute hypoxia-induced exercise intolerance and mitochondrial impairments in rats.
    Authors: Zou D, Liu P, Chen K, Xie Q, Liang X, Bai Q, Zhou Q, Liu K, Zhang T, Zhu J, Mi M
    PLoS ONE, 2015-04-28;10(4):e0124727.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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