Human/Mouse/Rat p38 alpha Antibody Summary
Met1-Ser360
Accession # Q16539
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human, Mouse, and Rat p38 alpha by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.5 µg/mL Rabbit Anti-Human/Mouse/Rat p38a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8691) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band for p38a was detected at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Human p38 alpha by Western Blot. Western blot shows recombinant human p38 beta, p38 gamma, p38 delta and Recombinant Human Active p38 alpha (5477-KS) (2 ng/lane). PVDF membrane was probed with 0.5 µg/mL Rabbit Anti-Human/Mouse/Rat p38 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8691) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Orexin B, p38 alpha, & Integrin beta 1 in Mouse Brainstem. Orexin B, p38a, and Integrin beta 1 were detected in perfusion fixed frozen sections of mouse brainstem using Human Orexin B Monoclonal Antibody (red; MAB734), Rabbit Anti-Human/Mouse/Rat p38a Antigen Affinity-purified Polyclonal Antibody (green; Catalog # AF8691), and Mouse Integrin beta 1 Antigen Affinity-purified Polyclonal Antibody (blue; AF2405). Tissue was incubated with primary antibodies overnight at 4 °C. Tissue was stained using NorthernLights™ 493-conjugated Anti-Rabbit IgG Secondary Antibody (green; NL006), NorthernLights 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007), and NorthernLights 637-conjugated Anti-Goat IgG Secondary Antibody (blue; NL002). The image of Integrin beta 1 is pseudo-colored for presentation. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
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Detection of Human and Mouse p38 alpha by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for p38 alpha at approximately 43 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse/Rat p38 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8691). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Western Blot Shows Human p38 alpha Specificity by Using Knockout Cell Line. Western blot shows lysates of HEK293T human embryonic kidney parental cell line and p38a knockout HEK293T cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat p38a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8691) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for p38a at approximately 38 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of p38 alpha in Mouse Brain Cerebellum. p38 alpha was detected in perfusion fixed paraffin-embedded sections of Mouse Brain Cerebellum using Rabbit Anti-Human/Mouse/Rat p38 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8691) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in Purkinje neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: p38 alpha
The p38 Mitogen-activated Protein Kinases (MAPKs) are a family of four related Ser/Thr kinases activated by proinflammatory cytokines and environmental stresses, such as UV irradiation and heat shock. Stress signals are delivered to this cascade by members of small GTPases of the Rho family (Rac, Rho, Cdc42). p38 MAPK is involved in the regulation of Hsp27 and MAPKAP-2 and several transcription factors including ATF2, STAT1, and indirectly CREB via activation of MSK1. The p38 MAPK protein also plays a role in cell differentiation, autophagy and apoptosis. Mkk3 and SEK can activate p38 MAPK by phosphorylation at Thr180 and Tyr182, which in turn activates the MAPKAP kinase 2 and regulating phosphorylation of ATF2, Mac and MEF2.
Product Datasheets
Citations for Human/Mouse/Rat p38 alpha Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
11
Citations: Showing 1 - 10
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Contribution of Increased Expression of Yin Yang 2 to Development of Cardiomyopathy
Authors: Zhang Y, Beketaev I, Segura AM et al.
Front Mol Biosci
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Venlafaxine, an anti-depressant drug, induces apoptosis in MV3 human melanoma cells through JNK1/2-Nur77 signaling pathway
Authors: Ting Niu, Zhiying Wei, Jiao Fu, Shu Chen, Ru Wang, Yuya Wang et al.
Frontiers in Pharmacology
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Combined anticancer therapy with imidazoacridinone C-1305 and paclitaxel in human lung and colon cancer xenografts-Modulation of tumour angiogenesis
Authors: M ?witalska, B Filip-Psur, M Milczarek, M Psurski, A Moszy?ska, AM D?browska, M Gawro?ska, K Krzymi?ski, M Bagi?ski, R Bartoszews, J Wietrzyk
Oncogene, 2022-06-14;0(0):.
Species: Xenograft
Sample Types: Tissue Homogenates
Applications: Simple Western -
Resveratrol and exercise combined to treat functional limitations in late life: A pilot randomized controlled trial
Authors: SA Harper, JR Bassler, S Peramsetty, Y Yang, LM Roberts, D Drummer, RT Mankowski, C Leeuwenbur, K Ricart, RP Patel, MM Bamman, SD Anton, BC Jaeger, TW Buford
Exp Gerontol, 2020-10-15;0(0):111111.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Role of Alterations in Protein Kinase p38&gamma in the Pathogenesis of the Synaptic Pathology in Dementia With Lewy Bodies and &alpha-Synuclein Transgenic Models
Authors: M Iba, C Kim, J Florio, M Mante, A Adame, E Rockenstei, S Kwon, R Rissman, E Masliah
Front Neurosci, 2020-03-31;14(0):286.
Species: Human, Mouse
Sample Types: Whole Tissue
Applications: IHC -
Selective p38? MAP kinase/MAPK14 inhibition in enzymatically-modified LDL-stimulated human monocytes: implications for atherosclerosis
Authors: Michael Torzewski
FASEB J., 2016-11-08;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: IHC-P -
p38 MAPK regulates the Wnt inhibitor Dickkopf-1 in osteotropic prostate cancer cells
Authors: A J Browne, A Göbel, S Thiele, L C Hofbauer, M Rauner, T D Rachner
Cell Death & Disease
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Rapid and Sensitive Lentivirus Vector-Based Conditional Gene Expression Assay to Monitor and Quantify Cell Fusion Activity
Authors: Manuel A. F. V. Gonçalves, Josephine M. Janssen, Maarten Holkers, Antoine A. F. de Vries
PLoS ONE
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E-selectin regulates gene expression in metastatic colorectal carcinoma cells and enhances HMGB1 release.
Authors: Aychek T, Miller K, Sagi-Assif O, Levy-Nissenbaum O, Israeli-Amit M, Pasmanik-Chor M, Jacob-Hirsch J, Amariglio N, Rechavi G, Witz IP
Int. J. Cancer, 2008-10-15;123(8):1741-50.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Interaction between the CCR5 chemokine receptors and microbial HSP70.
Authors: Whittall T, Wang Y, Younson J, Kelly C, Bergmeier L, Peters B, Singh M, Lehner T
Eur. J. Immunol., 2006-09-01;36(9):2304-14.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Cell differentiation versus cell death: extracellular glucose is a key determinant of cell fate following oxidative stress exposure.
Authors: Poulsen RC, Knowles HJ, Carr AJ et al.
Cell Death Dis
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Very good detection peaks while using Simple Western, as indicated.
