Human/Mouse/Rat p38 gamma Antibody

Detection of Human/Mouse/Rat p38 gamma by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line myoblast cell line and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat p38 gamma Monoclonal Antibody (Catalog # MAB1347) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human p38 beta, p38 gamma, p38d and p38a (2 ng/lane) were included. A specific band for native p38 gamma was detected at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

p38 gamma in MCF‑7 and MOLT‑4 Human Cell Lines. p38 gamma was detected in immersion fixed MCF-7 human breast cancer cell line (positive control, left panel) and MOLT-4 human acute lymphoblastic leukemia cell line (negative control, right panel) using Mouse Anti-Human/Mouse/Rat p38 gamma Monoclonal Antibody (Catalog # MAB1347) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm in MCF-7 cells. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Mouse and Rat p38 gamma by Simple Western^TM. Simple Western lane view shows lysates of C2C12 mouse myoblast cell line and PC‑12 rat adrenal pheochromocytoma cell line, loaded at 0.5 mg/mL. A specific band was detected for p38 gamma at approximately 46 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat p38 gamma Monoclonal Antibody (Catalog # MAB1347). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Mouse p38 gamma by Western Blot Muscle-specific deletion of the p38 alpha, p38 beta or p38 gamma gene does not affect endurance exercise-induced fiber-type transformation. Wild type, p38 alpha, p38 beta, and p38 gamma MKO mice were subjected to 4 weeks of voluntary running (Ex) or sedentary cage activity (Sed) followed by fiber-type and immunoblot analyses in plantaris muscles. A) PCR of genomic DNA with the appropriate primers (see Materials and Methods) for the loxP flanked p38 alleles in wild type (WT), heterozygous (+/−) and homozygous (−/−) mice with loxP-flanked p38 alleles. Only the homozygous mice with the Cre transgene (not shown) were considered p38 muscle-specific knockout (MKO) mice; B) Immunoblot analysis for p38 alpha and p38 gamma protein in plantaris muscles of p38 MKO mice in comparison with the wild type littermates. alpha -tubulin was used as a loading control; and C) Images of immunofluorescence staining of plantaris muscle sections with antibodies against myosin heavy chain IIb (Green), IIa (Blue) and I (Red). Appreciable increases in the percentage of type IIa fibers with concurrent decreases in type IIb fibers are noted in Ex group compared with Sed group among all four genetic backgrounds. The quantitative data is presented in Table 1. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/19936205), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse p38 gamma by Western Blot Muscle-specific deletion of the p38 alpha, p38 beta or p38 gamma gene does not affect endurance exercise-induced fiber-type transformation. Wild type, p38 alpha, p38 beta, and p38 gamma MKO mice were subjected to 4 weeks of voluntary running (Ex) or sedentary cage activity (Sed) followed by fiber-type and immunoblot analyses in plantaris muscles. A) PCR of genomic DNA with the appropriate primers (see Materials and Methods) for the loxP flanked p38 alleles in wild type (WT), heterozygous (+/−) and homozygous (−/−) mice with loxP-flanked p38 alleles. Only the homozygous mice with the Cre transgene (not shown) were considered p38 muscle-specific knockout (MKO) mice; B) Immunoblot analysis for p38 alpha and p38 gamma protein in plantaris muscles of p38 MKO mice in comparison with the wild type littermates. alpha -tubulin was used as a loading control; and C) Images of immunofluorescence staining of plantaris muscle sections with antibodies against myosin heavy chain IIb (Green), IIa (Blue) and I (Red). Appreciable increases in the percentage of type IIa fibers with concurrent decreases in type IIb fibers are noted in Ex group compared with Sed group among all four genetic backgrounds. The quantitative data is presented in Table 1. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/19936205), licensed under a CC-BY license. Not internally tested by R&D Systems.