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Human/Mouse/Rat p38 gamma Antibody

Catalog #: AF1347
8 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1347
AF1347-SP
Detection of Mouse/Rat p38 gamma by Western Blot.
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Citations (8)
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Human/Mouse/Rat p38 gamma Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat p38 gamma in Western blots. The antibody is highly specific for p38 gamma and in Western blots, shows extremely weak cross-reactivity with recombinant p38 alpha, p38 beta, or p38δ after prolonged exposure to film.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human p38 gamma
Accession # P53778
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
Simple Western
2 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
Immersion fixed paraffin-embedded sections of human breast, human skin cancer tissue, and mouse skeletal muscle
Immunocytochemistry
3-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse/Rat p38? antibody by Western Blot. View Larger

Detection of Mouse/Rat p38 gamma by Western Blot. Western blot shows lysates of PC-12 rat adrenal pheochromocytoma cell line, C6 rat glioma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.2 µg/mL Rabbit Anti-Human/Mouse/Rat p38 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1347) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for p38 gamma was detected at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry p38? antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC). View Larger

p38 gamma in MCF‑7 Human Cell Line. p38 gamma was detected in immersion fixed MCF-7 human breast cancer cell line using Rabbit Anti-Human/Mouse/Rat p38 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1347) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Western Blot Detection of human p38? antibody by Western Blot. View Larger

Detection of human p38 gamma by Western Blot. Western blot shows recombinant human p38 beta, p38 gamma, p38d and p38a (1 ng/lane). PVDF membrane was probed with 0.2 µg/mL Rabbit Anti-Human/Mouse/Rat p38 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1347) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for p38 gamma was detected at approx-imately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Mouse and Rat p38? antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Mouse and Rat p38 gamma by Simple WesternTM. Simple Western lane view shows lysates of C2C12 mouse myoblast cell line and PC‑12 rat adrenal pheochromocytoma cell line, loaded at 0.2 mg/mL. A specific band was detected for p38 gamma at approximately 48 kDa (as indicated) using 2 µg/mL of Rabbit Anti-Human/Mouse/Rat p38 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1347). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Western Blot Detection of Porcine p38 gamma/SAPK3 by Western Blot View Larger

Detection of Porcine p38 gamma/SAPK3 by Western Blot MAPK expression in normal and diabetic rat inferior vena cava. Immunoblot analyses indicating protein content of A) p44/42-MAPK, B) p38-MAPK, and C) JNK-MAPK in venae cavae from non-diabetic lean Zucker (LNZ) and diabetic obese syndrome X Zucker (OSXZ) rats. * Significantly different from the non-diabetic venae cavae, (P < 0.05). n = 6/group. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/16961925), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Rat p38 gamma/SAPK3 by Western Blot View Larger

Detection of Rat p38 gamma/SAPK3 by Western Blot MAPK expression in normal and diabetic rat inferior vena cava. Immunoblot analyses indicating protein content of A) p44/42-MAPK, B) p38-MAPK, and C) JNK-MAPK in venae cavae from non-diabetic lean Zucker (LNZ) and diabetic obese syndrome X Zucker (OSXZ) rats. * Significantly different from the non-diabetic venae cavae, (P < 0.05). n = 6/group. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/16961925), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: p38 gamma

p38 mitogen-activated protein kinase (MAPK)gamma, also known as p38 gamma, MAPK12, SAPK3 and ERK6 is a serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays a role in myoblast differentiation and also in the
down-regulation of cyclin D1 in response to hypoxia in adrenal cells.

Entrez Gene IDs
6300 (Human)
Alternate Names
EC 2.7.11; ERK3; ERK-6; ERK6MAPK 12; Extracellular signal-regulated kinase 6; MAP kinase 12; MAP kinase p38 gamma; MAPK12; mitogen-activated protein kinase 12; mitogen-activated protein kinase 3; Mitogen-activated protein kinase p38 gamma; p38 gamma; p38gamma; PRKM12; SAPK-3; SAPK3EC 2.7.11.24; Stress-activated protein kinase 3

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Citations for Human/Mouse/Rat p38 gamma Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Treatment of epilepsy using a targeted p38gamma kinase gene therapy
    Authors: N Morey, M Przybyla, J van der Ho, YD Ke, F Delerue, J van Eersel, LM Ittner
    Science Advances, 2022-12-02;8(48):eadd2577.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. Role of Alterations in Protein Kinase p38&gamma in the Pathogenesis of the Synaptic Pathology in Dementia With Lewy Bodies and &alpha-Synuclein Transgenic Models
    Authors: M Iba, C Kim, J Florio, M Mante, A Adame, E Rockenstei, S Kwon, R Rissman, E Masliah
    Front Neurosci, 2020-03-31;14(0):286.
    Species: Human, Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Impact of p38? Mitogen-Activated Protein Kinase (MAPK) on MDA-MB-231 Breast Cancer Cells Using Metabolomic Approach
    Authors: H Chen, X Wang, F Guo, P Li, D Peng, J He
    Int. J. Biochem. Cell Biol., 2018-11-14;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  4. Potential Neuroprotective Effects of an LSD1 Inhibitor in Retinal Ganglion Cells via p38 MAPK Activity
    Invest. Ophthalmol. Vis. Sci, 2016-11-01;57(14):6461-6473.
    Species: Rat
    Sample Types: Whole Cells
    Applications: IHC-Fr
  5. Kif13b Regulates PNS and CNS Myelination through the Dlg1 Scaffold
    Authors: R Noseda, M Guerrero-V, V Alberizzi, SC Previtali, DL Sherman, M Palmisano, RL Huganir, KA Nave, A Cuenda, ML Feltri, PJ Brophy, A Bolino
    PLoS Biol, 2016-04-12;14(4):e1002440.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  6. p38gamma MAPK Is a Therapeutic Target for Triple-Negative Breast Cancer by Stimulation of Cancer Stem-Like Cell Expansion.
    Authors: Qi X, Yin N, Ma S, Lepp A, Tang J, Jing W, Johnson B, Dwinell M, Chitambar C, Chen G
    Stem Cells, 2015-06-23;33(9):2738-47.
    Species: Human, Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  7. Differential tissue expression and activation of p38 MAPK alpha, beta, gamma, and delta isoforms in rheumatoid arthritis.
    Authors: Korb A, Tohidast-Akrad M, Cetin E, Axmann R, Smolen J, Schett G
    Arthritis Rheum., 2006-09-01;54(9):2745-56.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: IHC, Immunoprecipitation, Western Blot
  8. p38 MAPK regulates the Wnt inhibitor Dickkopf-1 in osteotropic prostate cancer cells
    Authors: A J Browne, A Göbel, S Thiele, L C Hofbauer, M Rauner, T D Rachner
    Cell Death & Disease

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