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Human/Mouse/Rat PAK6 Antibody

Catalog #: AF4265
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4265
AF4265-SP
Detection of Human/Mouse/Rat PAK6 by Western Blot.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat PAK6 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects endogenous human, mouse and rat PAK6 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human PAK6
Met1-Arg137
Accession # Q9NQU5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/Rat PAK6 antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat PAK6 by Western Blot. Western blot shows lysates of HT-29 human colon adenocarcinoma cell line, A431 human epithelial carcinoma cell line, KG-1 human acute myelogenous leukemia cell line, and CH-1 mouse B cell lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Human/Mouse/Rat PAK6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4265) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PAK6 at approximately 75 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 3.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PAK6

As downstream targets of the Rho GTPases, the p21-activated kinase (PAK) family of serine/threonine kinases regulates the organization of the actin cytoskeleton in mammalian cells. The PAK family is structurally categorized in two groups, each with three members: group I PAK1-3; and group II PAK4-6. PAK6 is expressed most highly in brain and testes, with lower levels in multiple tissues. Both MKK6 and p38 MAPK activate PAK6, suggesting a role for this kinase in the cellular stress response.

Long Name
p21/Cdc42/Rac1-activated Kinase 6
Entrez Gene IDs
56924 (Human); 214230 (Mouse); 296078 (Rat)
Alternate Names
EC 2.7.11; p21 protein (Cdc42/Rac)-activated kinase 6; p21(CDKN1A)-activated kinase 6; p21-activated kinase 6; p21-activated protein kinase 6; PAK-5; PAK5EC 2.7.11.1; PAK6; PAK-6; serine/threonine-protein kinase PAK 6

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Citations for Human/Mouse/Rat PAK6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. INPP4B suppresses prostate cancer cell invasion.
    Authors: Hodgson, Myles C, Deryugina, Elena I, Suarez, Egla, Lopez, Sandra M, Lin, Dong, Xue, Hui, Gorlov, Ivan P, Wang, Yuzhuo, Agoulnik, Irina U
    Cell Commun Signal, 2014-09-25;12(0):61.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Diversity of antigen-specific responses induced in vivo with CTLA-4 blockade in prostate cancer patients.
    Authors: Kwek, Serena S, Dao, Vinh, Roy, Ritu, Hou, Yafei, Alajajian, David, Simko, Jeffrey, Small, Eric J, Fong, Lawrence
    J Immunol, 2012-09-05;189(7):3759-66.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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