Human/Mouse/Rat Park7/DJ-1 Antibody

Catalog #: MAB39951 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB39951
MAB39951-SP

Detection of Human, Mouse, and Rat Park7/DJ‑1 by Western Blot.

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All Park7/DJ-1 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Park7/DJ-1 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat PARK-7 in Western blots. In sandwich immunoassays, it is specific for human PARK-7.

Source

Recombinant Monoclonal Mouse IgG_2A Clone # 925805R

Purification

Protein A or G purified from cell culture supernatant

Immunogen

E. coli-derived recombinant human PARK-7
Ala2-Asp189
Accession # Q99497

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

ELISA

This antibody functions as an ELISA detection antibody for Human Park7//DJ‑1 when paired with Mouse Anti-Human Park7/DJ‑1 Monoclonal Antibody (Catalog # MAB39952).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Park7/DJ-1 DuoSet ELISA Kit (Catalog # DY3995) for convenient development of a sandwich ELISA.

Immunocytochemistry

8-25 µg/mL

See below

Knockout Validated

Park7/DJ‑1 is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in Park7/DJ‑1 knockout HEK293T cell line.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat Park7/DJ‑1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MEF mouse embryonic feeder cells, and C6 rat glioma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Park7/DJ-1 Monoclonal Antibody (Catalog # MAB39951) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Park7/DJ-1 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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Park7/DJ‑1 in HeLa Human Cell Line. Park7/DJ-1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat Park7/DJ-1 Monoclonal Antibody (Catalog # MAB39951) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Knockout Validated

Western Blot Shows Human Park7/DJ-1 Antibody Specificity by Using Knockout Cell Line.

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Western Blot Shows Human Park7/DJ‑1 Specificity by Using Knockout Cell Line. Western blot shows lysates of HEK293T human embryonic kidney parental cell line and Park7 knockout HEK293T cell line (KO). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Park7/DJ-1 Monoclonal Antibody (Catalog # MAB39951) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Park7/DJ-1 at approximately 23 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

ELISA

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Human Park7/DJ‑1 ELISA Standard Curve. Recombinant Human Park7/DJ-1 protein was serially diluted 2-fold and captured by Mouse Anti-Human Park7/DJ-1 Monoclonal Antibody (Catalog # MAB39952) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human/Mouse/Rat Park7/DJ-1 Monoclonal Antibody (Catalog # MAB39951) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Park7/DJ-1

Park7, also known as DJ-1, is a cytoplasmic protein that belongs to the ThiJ/Pfp1/DJ-1 superfamily of highly conserved proteins that function as protein chaperones, catalases, proteases and kinases. Park7 is widely expressed in the brain as well as in peripheral tissues. It exists as a homodimer that can be localized in the cytoplasm, nucleus and mitochondria. Park7 is a redox-sensitive protein that has been ascribed various functions including that as a redox sensor and antioxidant protein. Mutations in Park7 are associated with a small percentage of hereditary early onset Parkinson’s disease. Human and mouse Park7 share 92% amino acid sequence identity.

Long Name

Parkinson Disease 7

Entrez Gene IDs

11315 (Human); 57320 (Mouse); 117287 (Rat)

Alternate Names

DJ1; DJ-1; DJ1FLJ34360; EC 3.4; FLJ27376; FLJ92274; Oncogene DJ1; Park7; Parkinson disease (autosomal recessive, early onset) 7; Parkinson disease protein 7; protein DJ-1