Human/Mouse/Rat Parvalbumin alpha Antibody Summary
Ser2-Ser110
Accession # P20472
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human/Mouse/Rat Parvalbumin alpha by Western Blot. Western blot shows lysates of human, mouse, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Parvalbumin a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5058) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Parvalbumin a at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
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Parvalbumin alpha in Human Brain. Parvalbumin a was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using 5 µg/mL Sheep Anti-Human/Mouse/Rat Parvalbumin a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5058) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Detection of Rat Parvalbumin alpha by Simple WesternTM. Simple Western lane view shows lysates of rat brain tissue, loaded at 0.2 mg/mL. A specific band was detected for Parvalbumin a at approximately 15 kDa (as indicated) using 50 µg/mL of Sheep Anti-Human/Mouse/Rat Parvalbumin a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5058) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Detection of Human and Mouse Parvalbumin alpha by Simple WesternTM. Simple Western lane view shows lysates of mouse brain (cortex) and human brain (cerebellum), loaded at 0.2 mg/mL. A specific band was detected for Parvalbumin a at approximately 14 and 17 kDa (as indicated) using 50 µg/mL of Sheep Anti-Human/Mouse/Rat Parvalbumin a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5058) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Parvalbumin alpha
Parvalbumin (Parvalbumin alpha ) is a 12 kDa member of the parvalbumin family of Ca++-binding proteins. In human, it is expressed in intrafusal muscle fibers, plus GABAergic interneurons and cerebellar Purkinje and basket cells. It presumably acts as a Ca++ buffer that shortens the duration of fiber contraction. Human Parvalbumin is 110 amino acids (aa) in length. It contains two EF-hand domains (aa 39-74 and 78-110) that bind calcium. There are three potential isoform variants. One shows an alternate start site at Met33, a second shows a six aa substitution for the C-terminal nine amino acids and a third shows a deletion of Gly99-Val100. Human Parvalbumin alpha is 51% aa identical to human Parvalbumin beta and is 87% plus 92% aa identical to mouse and rat Parvalbumin, respectively.
Product Datasheets
Citations for Human/Mouse/Rat Parvalbumin alpha Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
14
Citations: Showing 1 - 10
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Layer 4 Gates Plasticity in Visual Cortex Independent of a Canonical Microcircuit
Authors: Frantz MG, Crouse EC, Sokhadze G et al.
Curr. Biol.
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In vivo survival and differentiation of Friedreich ataxia iPSC-derived sensory neurons transplanted in the adult dorsal root ganglia
Authors: Viventi S, Frausin S, Howden SE et al.
Stem cells translational medicine
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Isolated loss of the AUTS2 long isoform, brain-wide or targeted to Calbindin -lineage cells, generates a specific suite of brain, behavioral and molecular pathologies
Authors: Song, Y;Seward, CH;Chen, CY;LeBlanc, A;Leddy, AM;Stubbs, L;
bioRxiv : the preprint server for biology
Species: Transgenic Mouse
Sample Types: Whole Tissue
Applications: Immunohistochemistry -
Knockdown of GABAA alpha3 subunits on thalamic reticular neurons enhances deep sleep in mice
Authors: DS Uygun, C Yang, ER Tilli, F Katsuki, EL Hodges, JT McKenna, JM McNally, RE Brown, R Basheer
Nature Communications, 2022-04-26;13(1):2246.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Aberrant Gamma-Band Oscillations in Mice with Vitamin D Deficiency: Implications on Schizophrenia and its Cognitive Symptoms
Authors: S Yu, M Park, J Kang, E Lee, J Jung, T Kim
Journal of personalized medicine, 2022-02-20;12(2):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Pre-treatment with microRNA-181a Antagomir Prevents Loss of Parvalbumin Expression and Preserves Novel Object Recognition Following Mild Traumatic Brain Injury
Authors: Brian B. Griffiths, Peyman Sahbaie, Anand Rao, Oiva Arvola, Lijun Xu, Deyong Liang et al.
NeuroMolecular Medicine
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Nogo receptor 1 is expressed by nearly all retinal ganglion cellsPV expression in Figure 3
Authors: AM Solomon, T Westbrook, GD Field, AW McGee
PLoS ONE, 2018-05-16;13(5):e0196565.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Expression of the onconeural protein CDR1 in cerebellum and ovarian cancer
Authors: C Totland, T Kråkenes, K Mazengia, M Haugen, C Vedeler
Oncotarget, 2018-05-08;9(35):23975-23986.
Species: Human, Mouse, Rat
Sample Types: Whole Tissue
Applications: IHC -
Phenotypic and Functional Characterization of Peripheral Sensory Neurons derived from Human Embryonic Stem Cells
Authors: AJ Alshawaf, S Viventi, W Qiu, G D'Abaco, B Nayagam, M Erlichster, G Chana, I Everall, J Ivanusic, E Skafidas, M Dottori
Sci Rep, 2018-01-12;8(1):603.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Nogo Receptor 1 Confines a Disinhibitory Microcircuit to the Critical Period in Visual Cortex
Authors: Aaron W McGee
J. Neurosci., 2016-10-26;36(43):11006-11012.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Unbiased classification of sensory neuron types by large-scale single-cell RNA sequencing.
Authors: Usoskin D, Furlan A, Islam S, Abdo H, Lonnerberg P, Lou D, Hjerling-Leffler J, Haeggstrom J, Kharchenko O, Kharchenko P, Linnarsson S, Ernfors P
Nat Neurosci, 2014-11-24;18(1):145-53.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Plasticity of binocularity and visual acuity are differentially limited by nogo receptor.
Authors: Stephany C, Chan L, Parivash S, Dorton H, Piechowicz M, Qiu S, McGee A
J Neurosci, 2014-08-27;34(35):11631-40.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Neuronal cell type-specific alternative splicing is regulated by the KH domain protein SLM1.
Authors: Iijima, Takatosh, Iijima, Yoko, Witte, Harald, Scheiffele, Peter
J Cell Biol, 2014-01-27;204(3):331-42.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Parvalbumin-containing chandelier and basket cell boutons have distinctive modes of maturation in monkey prefrontal cortex.
Authors: Fish K, Hoftman G, Sheikh W, Kitchens M, Lewis D
J Neurosci, 2013-05-08;33(19):8352-8.
Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
Sample Types: Whole Tissue
Applications: IHC
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