Human/Mouse/Rat PDCD6 Antibody Summary
Met1-Val189
Accession # O75340
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human, Mouse, and Rat PDCD6 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Hepa 1-6 mouse hepatoma cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human/Mouse/Rat PDCD6 Monoclonal Antibody (Catalog # MAB6015) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PDCD6 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PDCD6
PDCD6 (Programmed cell death protein 6; also ALG-2 and apoptosis-linked gene 2 protein) is a 22 kDa member of the penta-EF hand protein family of molecules. It is ubiquitously expressed, and performs multiple functions. PDCD6 is contextually linked to apoptosis, proliferation, membrane fusion and vesicle trafficking. Human PDCD6 is 191 amino acids (aa) in length. Although it contains five EF-hand domains, only domains #1 and #3 are considered functional (aa 23-58 and 90‑125). There is one splice form (ALG-2.1) that shows a deletion of Gly121Phe122. This form typically represents about 1/3 of total cellular PDCD6. PDCD6/ALG-2 will homodimerize, and heterodimerize with ALG-2.1. Notably, there are classes of cytosolic proteins that bind PDCD6, but not ALG-2.1, accounting for differences in function. Both human PDCD6 isoforms share 99% aa identity with their mouse counterparts.
Product Datasheets
Citation for Human/Mouse/Rat PDCD6 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Novel autophagy inducers by accelerating lysosomal clustering against Parkinson's disease
Authors: Date, Y;Sasazawa, Y;Kitagawa, M;Gejima, K;Suzuki, A;Saya, H;Kida, Y;Imoto, M;Itakura, E;Hattori, N;Saiki, S;
eLife
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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