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Human/Mouse/Rat Phospho-p38 MAP Kinase (T180/Y182) Antibody

Catalog #: AF869
15 Citations 1 Review Datasheet / COA / SDS

Discontinued Product

AF869 has been discontinued.
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Detection of Human Phospho-p38 MAP Kinase (T180/Y182) by Western Blot.
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Product Details
Citations (15)
FAQs
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Phospho-p38 MAP Kinase (T180/Y182) Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse and rat Phospho-p38 alpha  MAP Kinase when dually phosphorylated at T180/Y182. May also recognize the related phosphorylated isoforms p38 beta, p38 gamma and p38δ.
Source
Polyclonal Rabbit IgG
Purification
Antigen and protein A Affinity-purified
Immunogen
Phosphopeptide containing human, rat and mouse p38 alpha T180/Y182 site
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
5 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Phospho-p38 MAP Kinase (T180/Y182) antibody by Western Blot. View Larger

Detection of Human Phospho-p38 MAP Kinase (T180/Y182) by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 20 ng/mL Recombinant Human IL-1 beta (Catalog # 201-LB) for for the indicated times. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/ Rat Phospho-p38 MAP Kinase (T180/Y182) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF869), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-p38 MAP Kinase (T180/Y182) at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Phospho-p38 (T180/Y182) antibody in Mouse Embryo (13 d by Immunohistochemistry (IHC-Fr).p by Immunohistochemistry (IHC-Fr).c by Immunohistochemistry (IHC-Fr).) by Immunohistochemistry (IHC-Fr). View Larger

Phospho-p38 (T180/Y182) in Mouse Embryo (13 d.p.c.). p38 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Rabbit Anti-Human/Mouse/Rat Phospho-p38 MAP Kinase (T180/Y182) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF869) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific staining was localized to developing cartilage. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Simple Western Detection of Human Phospho-p38 MAP Kinase (T180/Y182) antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human Phospho-p38 MAP Kinase (T180/Y182) by Simple WesternTM. Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line untreated (-) or treated (+) with ultraviolet light (UV), loaded at 0.5 mg/mL. A specific band was detected for Phospho-p38 MAP Kinase (T180/Y182) at approximately 43 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-p38 MAP Kinase (T180/Y182) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF869). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: p38

The p38 Mitogen-activated Protein Kinases (MAPKs) are a family of four related Ser/Thr kinases activated by proinflammatory cytokines and environmental stresses, such as UV irradiation and heat shock. Stress signals are delivered to this cascade by members of small GTPases of the Rho family (Rac, Rho, Cdc42). p38 MAPK is involved in the regulation of Hsp27 and MAPKAP-2 and several transcription factors including ATF2, STAT1, and indirectly CREB via activation of MSK1. The p38 MAPK protein also plays a role in cell differentiation, autophagy and apoptosis. Mkk3 and SEK can activate p38 MAPK by phosphorylation at Thr180 and Tyr182, which in turn activates the MAPKAP kinase 2 and regulating phosphorylation of ATF2, Mac and MEF2.

Alternate Names
p38

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Citations for Human/Mouse/Rat Phospho-p38 MAP Kinase (T180/Y182) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. SENP3-mediated deSUMOylation of c-Jun facilitates microglia-induced neuroinflammation after cerebral ischemia and reperfusion injury
    Authors: Qian Xia, Meng Mao, Gaofeng Zhan, Zhenzhao Luo, Yin Zhao, Xing Li
    iScience
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Heme Oxygenase-1 Induction in Human BeWo Trophoblast Cells Decreases Toxoplasma gondii Proliferation in Association With the Upregulation of p38 MAPK Phosphorylation and IL-6 Production
    Authors: MPO Almeida, CM Mota, TWP Mineo, EAV Ferro, BF Barbosa, NM Silva
    Frontiers in Microbiology, 2021-04-12;12(0):659028.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. ADAM8 as a drug target in pancreatic cancer.
    Authors: Schlomann U, Koller G, Conrad C, Ferdous T, Golfi P, Garcia A, Hofling S, Parsons M, Costa P, Soper R, Bossard M, Hagemann T, Roshani R, Sewald N, Ketchem R, Moss M, Rasmussen F, Miller M, Lauffenburger D, Tuveson D, Nimsky C, Bartsch J
    Nat Commun, 2015-01-28;6(0):6175.
    Species: Human
    Sample Types: Whole Cells
    Applications: Blocking
  4. Molecular mechanisms underlying synergistic adhesion of sickle red blood cells by hypoxia and low nitric oxide bioavailability.
    Authors: Gutsaeva, Diana R, Montero-Huerta, Pedro, Parkerson, James B, Yerigenahally, Shobha D, Ikuta, Tohru, Head, C Alvin
    Blood, 2014-01-15;123(12):1917-26.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Pre-exposure of Mycobacterium tuberculosis-infected macrophages to crystalline silica impairs control of bacterial growth by deregulating the balance between apoptosis and necrosis.
    Authors: Chavez-Galan L, Ramon-Luing L, Torre-Bouscoulet L, Perez-Padilla R, Sada-Ovalle I
    PLoS ONE, 2013-11-22;8(11):e80971.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Mice with Tak1 deficiency in neural crest lineage exhibit cleft palate associated with abnormal tongue development.
    Authors: Song Z, Liu C, Iwata J, Gu S, Suzuki A, Sun C, He W, Shu R, Li L, Chai Y, Chen Y
    J Biol Chem, 2013-03-04;288(15):10440-50.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  7. Inhibition of Doxorubicin-Induced Senescence by PPARdelta Activation Agonists in Cardiac Muscle Cells: Cooperation between PPARdelta and Bcl6.
    Authors: Altieri P, Spallarossa P, Barisione C, Garibaldi S, Garuti A, Fabbi P, Ghigliotti G, Brunelli C
    PLoS ONE, 2012-09-25;7(9):e46126.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Dose-dependent changes in neuroinflammatory and arachidonic acid cascade markers with synaptic marker loss in rat lipopolysaccharide infusion model of neuroinflammation.
    BMC Neurosci, 2012-05-23;13(0):50.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  9. Glucose and collagen regulate human platelet activity through aldose reductase induction of thromboxane.
    Authors: Tang WH, Stitham J, Gleim S, Di Febbo C, Porreca E, Fava C, Tacconelli S, Capone M, Evangelista V, Levantesi G, Wen L, Martin K, Minuz P, Rade J, Patrignani P, Hwa J
    J. Clin. Invest., 2011-10-17;121(11):4462-76.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Osteoclast differentiation factor RANKL controls development of progestin-driven mammary cancer.
    Authors: Schramek D, Leibbrandt A, Sigl V, Kenner L, Pospisilik JA, Lee HJ, Hanada R, Joshi PA, Aliprantis A, Glimcher L, Pasparakis M, Khokha R, Ormandy CJ, Widschwendter M, Schett G, Penninger JM
    Nature, 2010-09-29;468(7320):98-102.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. The myeloperoxidase-derived oxidant HOSCN inhibits protein tyrosine phosphatases and modulates cell signalling via the mitogen-activated protein kinase (MAPK) pathway in macrophages.
    Authors: Lane AE, Tan JT, Hawkins CL
    Biochem. J., 2010-08-15;430(1):161-9.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. Colocalization of the VEGF-R2 and the common IL-3/GM-CSF receptor beta chain to lipid rafts leads to enhanced p38 activation.
    Authors: Saulle E, Riccioni R, Coppola S, Parolini I, Diverio D, Riti V, Mariani G, Laufer S, Sargiacomo M, Testa U
    Br. J. Haematol., 2009-02-24;145(3):399-411.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. MAP-ing glioma invasion: mitogen-activated protein kinase kinase 3 and p38 drive glioma invasion and progression and predict patient survival.
    Authors: Demuth T, Reavie LB, Rennert JL, Nakada M, Nakada S, Hoelzinger DB, Beaudry CE, Henrichs AN, Anderson EM, Berens ME
    Mol. Cancer Ther., 2007-04-03;6(4):1212-22.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  14. E2F1 modulates p38 MAPK phosphorylation via transcriptional regulation of ASK1 and Wip1.
    Authors: Hershko T, Korotayev K, Polager S, Ginsberg D
    J. Biol. Chem., 2006-08-15;281(42):31309-16.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  15. CCL3 (MIP-1alpha) induces in vitro migration of GM-CSF-primed human neutrophils via CCR5-dependent activation of ERK 1/2.
    Authors: Ottonello L, Montecucco F, Bertolotto M, Arduino N, Mancini M, Corcione A, Pistoia V, Dallegri F
    Cell. Signal., 2005-03-01;17(3):355-63.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Reviews for Human/Mouse/Rat Phospho-p38 MAP Kinase (T180/Y182) Antibody

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Human/Mouse/Rat Phospho-p38 MAP Kinase (T180/Y182) Antibody
By Anonymous on 10/26/2015
Application: WB Sample Tested: Min6 mouse insulinoma whole cell lysate Species: Mouse

Specificity: Reasonably specific
Sensitivity: Reasonably sensitive
Buffer: BSA
Dilution: 1:1000

Western Blot Human/Mouse/Rat Phospho-p38 MAP Kinase (T180/Y182) Antibody AF869