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Human/Mouse/Rat Phospho-WNK1 (T60) DuoSet IC ELISA

Catalog #: DYC4720-2 Datasheet / COA / SDS

Discontinued Product

DYC4720-2 has been discontinued.
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R&D Systems DuoSet ELISAs
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Summary Product Features Kit Content Other Reagents Required Product Datasheets Preparation and Storage Background

Human/Mouse/Rat Phospho-WNK1 (T60) DuoSet IC ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4 hours 40 minutes (after plate preparation)
Sample Volume Required
Cell lysates (100 µL)
Assay Range
15.6 - 1,000 pg/mL
Sufficient Materials
Kits available for two, five, or fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure phosphorylated human, mouse, and rat WNK1 (T60) in cell lysates. An immobilized capture antibody specific for WNK1 binds both phosphorylated and unphosphorylated WNK1. After washing away unbound material, a biotinylated detection antibody is used to detect only phosphorylated receptor, utilizing a standard HRP format.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Available in 2, 5, and 15- (96-well) plate pack sizes
  • Economical alternative to Western blot

Kit Content

  • Capture Antibody
  • Conjugated Detection Antibody
  • Calibrated Immunoassay Standard or Control
  • Streptavidin-HRP

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Product Datasheets

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Product Datasheet
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SDS

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: WNK1

WNK1 belongs to a novel family of serine/threonine protein kinases involved in regulating ion transport. The WNKs contain a cysteine instead of the usual lysine in their kinase activation domains. WNK1 mutations have been implicated in Pseudohypoaldosteronism type II (PHAII), an autosomal dominant disorder characterized by hypertension, increased renal salt absorption, and impaired potassium and hydrogen ion excretion.

Long Name:
WNK Lysine-deficient Protein Kinase 1
Entrez Gene IDs:
65125 (Human); 232341 (Mouse); 116477 (Rat)
Alternate Names:
EC 2.7.11; EC 2.7.11.1; Erythrocyte 65 kDa protein; HSAN2hereditary sensory neuropathy, type II; HSN2MGC163339; hWNK1; KDP; KDPMGC163341; KIAA0344; Kinase deficient protein; p65; PHA2C; PRKWNK1; PRKWNK1PSK; prostate-derived sterile 20-like kinase; Protein kinase lysine-deficient 1; Protein kinase with no lysine 1; protein kinase, lysine deficient 1; serine/threonine-protein kinase WNK1; WNK lysine deficient protein kinase 1; WNK1

FAQs

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