Home / PKA C (pan) / Human/Mouse/Rat PKA C Pan Specific Antibody

Human/Mouse/Rat PKA C Pan Specific Antibody

Catalog #: MAB4175
2 Citations Datasheet / COA / SDS
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MAB4175
MAB4175-SP

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Detection of Human/Mouse/Rat PKA C by Western Blot.
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Citations (2)
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Human/Mouse/Rat PKA C Pan Specific Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human PKA C alpha, C beta, and C gamma as well as endogenous human, mouse, and rat PKA C isoforms at 39-42 kDa using Western blot.
Source
Monoclonal Mouse IgG2A Clone # 485013
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human PKA C gamma
Met1-Phe351
Accession # ABM84853
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/Rat PKA C antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat PKA C by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, Neuro-2A mouse neuroblastoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL Human/Mouse/Rat PKA C Pan Specific Monoclonal Antibody (Catalog # MAB4175) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, Recombinant Human Active PKA C alpha (Catalog # 4268-KS), Recombinant Human Active PKA C beta (Catalog # 4596-KS), and recombinant human PKA C gamma (5 ng/lane) were included. A specific band for PKA C (pan) was detected at approximately 39 - 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Reconstitution Calculator

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PKA C (pan)

The cAMP-dependent protein kinase (PKA) holoenzyme is composed of two regulatory and two catalytic subunits, designated PKA R and PKA C, respectively. Three catalytic subunit isoforms, C alpha, C beta, and C gamma, have been identified. Upon PKA R subunit binding to the second messenger cAMP, active PKA C subunits are released, initiating a phosphorylation cascade that regulates such cellular functions as metabolism, ion transport, and gene transcription.

Long Name
cAMP-dependent Protein Kinase Catalytic Subunit
Alternate Names
CAFD1; PKA C; PKACA; PPNAD4

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Citations for Human/Mouse/Rat PKA C Pan Specific Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Neuromedin B regulates steroidogenesis, cell viability and apoptosis in rabbit Leydig cells
    Authors: Z Ma, J Guo, Y Zhang, Y Zhang, M Zhang, R Zong, F Chen, J Zhang
    Gen. Comp. Endocrinol., 2019-12-16;288(0):113371.
    Species: Rabbit
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Phosphodiesterase 3B (PDE3B) antagonizes the anti-angiogenic actions of PKA in human and murine endothelial cells
    Authors: JL MacKeil, P Brzezinska, J Burke-Klei, AL Theilmann, CJB Nicol, ML Ormiston, DH Maurice
    Cell. Signal., 2019-06-05;62(0):109342.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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