Human/Mouse/Rat PKA RI beta Antibody Summary
Met1-Val381
Accession # P31321
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human PKA RI beta by Western Blot. Western blot shows lysates of MDA-MB-468 human breast cancer cell line, Jurkat human acute T cell leukemia cell line, SW480 human colorectal adenocarcinoma cell line, Raji human Burkitt's lymphoma cell line, and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL Sheep Anti-Human/Mouse/Rat PKA RI beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4177) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for PKA RI beta was detected at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Human PKA RI beta by Western Blot. Western blot shows recombinant human PKA RIa, PKA RI beta, and PKA RII beta (5 ng/lane). PVDF membrane was probed with 1 µg/mL Sheep Anti-Human/ Mouse/Rat PKA RI beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4177) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for PKA RI beta was detected at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Human PKA RI beta by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for PKA RI beta at approximately 56 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human/Mouse/Rat PKA RI beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4177) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PKA RI beta
The cAMP-dependent protein kinase (PKA) holoenzyme is composed of two regulatory and two catalytic subunits, designated PKA R and PKA C, respectively. PKA R subunits exist as two classes, RI and RII, with each class containing two isoforms, alpha and beta. Upon PKA R subunit binding to the second messenger cAMP, active PKA C subunits are released, initiating a phosphorylation cascade that regulates such cellular functions as metabolism, ion transport, and gene transcription.
Product Datasheets
Citations for Human/Mouse/Rat PKA RI beta Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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A Bayesian Framework for Generalized Linear Mixed Modeling Identifies New Candidate Loci for Late-Onset Alzheimer's Disease
Authors: X Wang, VM Philip, G Ananda, CC White, A Malhotra, PJ Michalski, KRM Karuturi, SR Chintalapu, C Acklin, M Sasner, DA Bennett, PL De Jager, GR Howell, GW Carter
Genetics, 2018-03-05;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Isoform-specific subcellular localization and function of protein kinase A identified by mosaic imaging of mouse brain
Authors: Ronit Ilouz, Varda Lev-Ram, Eric A Bushong, Travis L Stiles, Dinorah Friedmann-Morvinski, Christopher Douglas et al.
eLife
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microRNA-208a in an early stage myocardial infarction rat model and the effect on cAMP-PKA signaling pathway
Authors: Gao Feng
Mol Med Rep, 2016-06-14;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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