Human/Mouse/Rat PKC gamma Antibody

Catalog #: AF5130 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5130
AF5130-SP

Detection of Human/Mouse/Rat PKC gamma by Western Blot.

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Human/Mouse/Rat PKC gamma Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects endogenous human, mouse and rat PKC gamma in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human PKC gamma
Arg621-Met697
Accession # P05129

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse/Rat PKC gamma by Western Blot. Western blot shows lysates of human, mouse, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PKC gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5130) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PKC gamma at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PKC gamma

PKC gamma (protein kinase C-gamma) is a 78-80 kDa member of the PKC subfamily, AGC Ser/Thr protein kinase family of enzymes. It is a Ca++-activated and phospholipid-dependent neuronal enzyme that catalyzes the phosphorylation of multiple proteins. Human PKC gamma is 697 amino acids (aa) in length. It contains two general regions: a lipid and Ca++-binding regulatory region (aa 35‑150 and 170‑260, respectively), and an ATP-binding catalytic region (aa 351‑614). PDK1-mediated phosphorylation of PKC gamma on Thr514 activates the enzyme. In addition, oxidative stress also mediates activation by inducing interchain disulfide-bond homodimerization. Over aa 621‑697, human PKC gamma is 99% and 95% aa identical to mouse and canine PKC gamma, respectively. PKC gamma is predominantly expressed in brain tissue.

Long Name

Protein Kinase C gamma

Entrez Gene IDs

5582 (Human); 18752 (Mouse); 24681 (Rat)

Alternate Names

EC 2.7.11; EC 2.7.11.13; MGC57564; PKC gamma; PKCC; PKCCSCA14; PKCG; PKC-gamma; PKCGprotein kinase C gamma type; PRKCG; protein kinase C, gamma; SCA14