Human/Mouse/Rat PKN2 Antibody Summary
Asp18-Asn207
Accession # Q16513
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human/Mouse/Rat PKN2 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, TF-1 human erythroleukemic cell line, C2C12 mouse myoblast cell line, NIH-3T3 mouse embryonic fibroblast cell line, L6 rat myoblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PKN2 Monoclonal Antibody (Catalog # MAB5686) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PKN2 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PKN2
Serine/Threonine Protein Kinase N2 (PKN2), also known as PRK2, is a cytoplasmic kinase that contains three HR1 repeats (aa 44‑295), one C2 domain (aa 330‑463), and one protein kinase domain (aa 657‑916). PKN2 is a downstream effector of RhoA and promotes the activation of several kinases involved in regulation of the actin cytoskeleton, cell cycle, and apoptosis. PKN2 cleavage during apoptosis generates a C‑terminal fragment that inhibits PDK1 and Akt. PKN2 is also important in the intracellular replication of hepatitis C virus and Yersinia pestis.
Product Datasheets
Citation for Human/Mouse/Rat PKN2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Disruption of pancreatic stellate cell myofibroblast phenotype promotes pancreatic tumor invasion
Authors: ER Murray, S Menezes, JC Henry, JL Williams, L Alba-Caste, P Baskaran, I Quétier, A Desai, JJT Marshall, I Rosewell, M Tatari, V Rajeeve, F Khan, J Wang, P Kotantaki, EJ Tyler, N Singh, CS Reader, EP Carter, K Hodivala-D, RP Grose, HM Kocher, N Gavara, O Pearce, P Cutillas, JF Marshall, AJM Cameron
Cell Reports, 2022-01-25;38(4):110227.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
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