Human/Mouse/Rat PLC-beta 4 Antibody

Catalog #: AF5128 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5128
AF5128-SP

Detection of Human/Mouse/Rat PLC-beta 4 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat PLC-beta 4 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects endogenous human, mouse, and, rat PLC-beta 4 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human PLC-beta 4
Lys75-Gln250
Accession # Q15147

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse/Rat PLC-beta 4 by Western Blot. Western blot shows lysates of DU145 human prostate carcinoma cell line, H4-II-E-C3 rat hepatoma cell line, and Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PLC-beta 4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5128) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PLC-beta 4 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PLC-beta 4

PLC-beta 4 (phospholipase C-beta 4; also PLCretA) is a 130 kDa member of the beta -type PLC isozyme family. It is notably expressed in cerebellum and retina. PLC-beta 4 is associated with rhodopsin family GPCRs, where activation of Gq alpha subunits initiates PLC-beta 4 hydrolysis of PIP2. Human PLC-beta 4 is 1175 amino acids (aa) in length. It contains a catalytic X-box (aa 313‑463) and Y-box (aa 565‑681), followed by a calcium-binding C2 domain (aa 688‑786). There are multiple isoforms. One shows an alternate start site at Met114, a second contains a 40 aa substitution for the C-terminal 22 aa, a third shows a 14 aa substitution for the N-terminal 167 aa, and other forms demonstrate combinations of these alternative splice forms. Over aa 75‑250, human PLC-beta 4 is 97% aa identical to mouse PLC-beta 4.

Long Name

Phospholipase C beta 4

Entrez Gene IDs

5332 (Human); 18798 (Mouse); 25031 (Rat)

Alternate Names

1-phosphatidyl-D-myo-inositol-4,5-bisphosphate; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta 4; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-4; beta 4 (1-Phosphatidylinositol-4,5-BisphosphatePhosphodiesterase Beta 4)); EC 3.1.4.11; FLJ16169; inositoltrisphosphohydrolase; monophosphatidylinositol phosphodiesterase; phosphoinositidase C; phosphoinositide phospholipase C; phospholipase C, beta 4; PLCB4; PLCbeta 4; PLC-beta 4; triphosphoinositide phosphodiesterase