Human/Mouse/Rat PP1 Catalytic Subunit Antibody

Catalog #: MAB3000
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3000
MAB3000-SP

Detection of Human/Mouse/Rat PP1ca by Western Blot.

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat PP1 Catalytic Subunit Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects endogenous human, mouse and rat PP1 alpha. Based on the amino acid homologies, the antibody is expected to cross‑react with PP1 beta and PP1 gamma.

Source

Monoclonal Mouse IgG_2A Clone # 319319

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human PP1 alpha catalytic subunit
Lys41-Pro298
Accession # P62136

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse/Rat PP1ca by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Nb2-11 rat lymphoma cell line, and Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PP1 Catalytic Subunit Monoclonal Antibody (Catalog # MAB3000) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for PP1 catalytic subunits at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PP1

Protein Phosphatase 1, also known as PP1 and PPP1, is an enzyme that removes phosphate groups attached to serine or threonine residues in proteins. The holoenzyme is composed of two subunits, a catalytic subunit (PP1ca) that is highly conserved throughout evolution, and a wide variety of regulatory subunits that target the enzyme to specific subcellular compartments and proteins. The catalytic subunit is ubiquitously expressed in tissues and cell lines.

Long Name

Protein Phosphatase 1

Entrez Gene IDs

5499 (Human); 19045 (Mouse); 24668 (Rat)

Alternate Names

PP1; PP1A;PP-1A;PP1alpha;PPP1A;PPP1CA;Serine/threonine-protein phosphatase;Serine/threonine-protein phosphatase PP1-alpha catalytic subunit

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse/Rat PP1 Catalytic Subunit Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Cell cycle dependent association of EBP50 with protein phosphatase 2A in endothelial cells.
Authors: Boratko A, Gergely P, Csortos C
PLoS ONE, 2012-04-16;7(4):e35595.
Species: Bovine
Sample Types: Cell Lysates
Applications: Western Blot
TIMAP is a positive regulator of pulmonary endothelial barrier function.
Authors: Csortos C, Czikora I, Bogatcheva NV, Adyshev DM, Poirier C, Olah G, Verin AD
Am. J. Physiol. Lung Cell Mol. Physiol., 2008-06-27;295(3):L440-50.
Species: Human
Sample Types: Whole Cells
Applications: ICC