Human/Mouse/Rat PP1 Catalytic Subunit Antibody

Catalog #: AF3000 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3000
AF3000-SP

Detection of Human/Mouse/Rat PP1 Catalytic Subunit, by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat PP1 Catalytic Subunit Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects endogenous human, mouse and rat PP1 alpha in Western blots. Based on the immunogen’s homology, the antibody is expected to cross‑react with PP1 beta and PP1 gamma.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human PP1 alpha Catalytic Subunit
Lys41-Pro298
Accession # P62136

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse/Rat PP1 Catalytic Subunit, by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PP1 Catalytic Subunit, Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3000) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PP1 at approximately 39 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PP1

Protein Phosphatase 1, also known as PP1 and PPP1, is an enzyme that removes phosphate groups attached to serine or threonine residues in proteins. The holoenzyme is composed of two subunits, a catalytic subunit (PP1ca) that is highly conserved throughout evolution, and a wide variety of regulatory subunits that target the enzyme to specific subcellular compartments and proteins. The catalytic subunit is ubiquitously expressed in tissues and cell lines.

Long Name

Protein Phosphatase 1

Entrez Gene IDs

5499 (Human); 19045 (Mouse); 24668 (Rat)

Alternate Names

PP1; PP1A;PP-1A;PP1alpha;PPP1A;PPP1CA;Serine/threonine-protein phosphatase;Serine/threonine-protein phosphatase PP1-alpha catalytic subunit