Human/Mouse/Rat PP1 Inhibitor-2 Antibody Summary
Met1-Ser205
Accession # P41236
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human/Mouse/Rat PP1 Inhibitor‑2 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line and mouse spleen tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat PP1 Inhibitor-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4719) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PP1 Inhibitor-2 at approximately 30 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
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Detection of Human PP1 Inhibitor‑2 by Simple WesternTM. Simple Western lane view shows lysates of Raji human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PP1 Inhibitor-2 at approximately 42 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat PP1 Inhibitor-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4719) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PP1 Inhibitor-2
Protein Phosphatase 1 Inhibitor-2 (PP1 Inhibitor-2), also called PP1 regulatory subunit 2 (PP1R2), I2, and IPP2, binds to PP1 through an RVxF consensus sequence. The complex inactivates PP1, preventing it from dephosphorylating substrates such as glycogen phosphorylase. Unlike PP1 Inhibitor-1, the binding of PP1 Inhibitor-2 is Protein Kinase A phosphorylation-independent. Dissociation of the PP1 Inhibitor-2 complex is enhanced by phosphorylation at T72 by GSK3, ERK, or CDK. Overexpression of PP1 Inhibitor-2 in mouse heart improves cardiac function without changing organ size.
Product Datasheets
Citations for Human/Mouse/Rat PP1 Inhibitor-2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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PP1 regulatory subunit NIPP1 regulates transcription of E2F1 target genes following DNA damage
Authors: S Hanaki, M Habara, T Masaki, K Maeda, Y Sato, M Nakanishi, M Shimada
Cancer Science, 2021-05-03;0(0):.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ChIP, Western Blot -
NMDAR-Activated PP1 Dephosphorylates GluN2B to Modulate NMDAR Synaptic Content
Authors: AM Chiu, J Wang, MP Fiske, P Hubalkova, L Barse, JA Gray, A Sanz-Cleme
Cell Rep, 2019-07-09;28(2):332-341.e5.
Species: Rat
Sample Types: Homogenized Tissue
Applications: Western Blot -
Cardiac-specific deletion of protein phosphatase 1 beta promotes increased myofilament protein phosphorylation and contractile alterations
Authors: Ruijie Liu, Robert N. Correll, Jennifer Davis, Ronald J. Vagnozzi, Allen J. York, Michelle A. Sargent et al.
Journal of Molecular and Cellular Cardiology
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