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Human/Mouse/Rat PP2A Catalytic Subunit Antibody

Catalog #: MAB1653 4 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1653
MAB1653-SP
Detection of Human and Mouse PP2A Catalytic Subunit, by Western Blot.
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat PP2A Catalytic Subunit Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects endogenous human, mouse, and rat PP2A in Western blots. Because PP2A-alpha and PP2A-beta catalytic subunits are identical in the region used for the immunogen, it is expected that both isoforms will be detected by the antibody.
Source
Monoclonal Rat IgG2B Clone # 234529
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human PP2A-alpha catalytic subunit
Trp209-Leu309
Accession # P67775
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse PP2A Catalytic Subunit, antibody by Western Blot. View Larger

Detection of Human and Mouse PP2A Catalytic Subunit, by Western Blot. Western blot shows lysates of U937 human histiocytic lymphoma cell line and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PP2A Catalytic Subunit, Monoclonal Antibody (Catalog # MAB1653) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for PP2A Catalytic Subunit, at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PP2A

Protein Phosphatase 2A (PP2A) dephosphorylates serine and threonine residues in proteins. This ubiquitously expressed enzyme plays a critical role in modulating cell survival, growth factor responses, and neurotransmission. Phosphorylation near the C-terminus at Y307 of the catalytic subunit decreases the phosphatase activity of PP2A and reduces its ability to bind to proteins such as CD28 and the glutamate receptor GluR5. Phosphorylation of PP2A at Y307 is highest during M-phase and lowest during S-phase of the cell cycle. Transient PP2A phosphorylation at this site is also observed in growth factor-stimulated cells.

Entrez Gene IDs
5515 (Human); 51792 (Mouse)
Alternate Names
EC 3.1.3.16; PP2A; PP2A-alpha; PP2Ac; PP2CA; PP2Calpha; PPP2CA; protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform; protein phosphatase 2, catalytic subunit, alpha isozyme; protein phosphatase 2A catalytic subunit, alpha isoform; Replication protein C; RP-C; serine/threonine protein phosphatase 2A, catalytic subunit, alpha isoform; serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform

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Reviews for Human/Mouse/Rat PP2A Catalytic Subunit Antibody

Average Rating: 4.3 (Based on 4 Reviews)

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Human/Mouse/Rat PP2A Catalytic Subunit Antibody
By Anonymous on 01/14/2021
Application: Microarrays Sample Tested: EDTA Plasma Species: Human
Other Human/Mouse/Rat PP2A Catalytic Subunit Antibody MAB1653
Human/Mouse/Rat PP2A Catalytic Subunit Antibody
By Anonymous on 11/20/2018
Application: MiAr Sample Tested: EDTA Plasma Species: Human
Human/Mouse/Rat PP2A Catalytic Subunit Antibody
By Anonymous on 11/08/2018
Application: Luminex Sample Tested: Serum Species: Human
Human/Mouse/Rat PP2A Catalytic Subunit Antibody
By Anonymous on 11/08/2018
Application: ELISA Sample Tested: Plasma,Serum Species: Human and Mouse