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Human/Mouse/Rat PP2C alpha /PPM1A Antibody

Catalog #: MAB4150
1 Citation Datasheet / COA / SDS
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MAB4150
MAB4150-SP
Detection of Human, Mouse, and Rat PP2C alpha /PPM1A by Western Blot.
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Human/Mouse/Rat PP2C alpha /PPM1A Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat PP2C alpha /PPM1A in Western blots. No cross-reactivity with recombinant human PP2C beta, ILKAP/PP2Cδ, or PP2C gamma is observed.
Source
Monoclonal Mouse IgG1 Clone # 498922
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human PP2C alpha /PPM1A
Met1-Gly324
Accession # P35813
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat PP2Ca/PPM1A antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat PP2C alpha /PPM1A by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, Neuro-2A mouse neuroblastoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat PP2Ca/ PPM1A Monoclonal Antibody (Catalog # MAB4150) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PP2Ca/PPM1A at approximately 41 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PP2C alpha/PPM1A

Protein phosphatase 2C alpha, also called PP2C alpha, Protein Phosphatase, Magnesium-dependent type 1A, and PPM1A, dephosphorylates proteins with some preference for phospho-threonine over phospho-serine residues (1). It is insensitive to the PP1 and PP2a inhibitors such as okadaic acid, (2) but has an absolute requirement for either magnesium or manganese ions (3) and can be activated by unsaturated fatty acids such as arachidonic and oleic acids (4). Overexpression of PP2C alpha causes G2/M cell cycle arrest and apoptosis that has been associated with the activation of p53 (5). Dephosphorylation of proteins in the nucleus by PP2C alpha is suspected to play a role in terminating or reducing the sensitivity of the responses to SMADS (6) and stress-activated proteins such as p38 and JNK (7).

References
  1. Donella-Deanna, A. et al. (1991) Biochimica et Biophysica Acta 1094:130.
  2. Bialojan, C. and A. Takai (1998) Biochem. J. 256:283.
  3. Cohen, P. Annu. Rev. Biochem. (1989) 58:453.
  4. Klumpp, S. et al. (1998) FEBS Let. 437:229.
  5. Ofek, P. et al. (2003) J. Biol. Chem. 278:14299.
  6. Lin, X. et al. (2006) Cell 125:915.
  7. Takekawa, M. et al. (1998) EMBO J. 17:4744.
Long Name
Protein Phosphatase 2C alpha
Entrez Gene IDs
5494 (Human); 19042 (Mouse); 24666 (Rat)
Alternate Names
EC 3.1.3.16; FLJ42306; MGC9201; MMPa-2; MPPa-1; phosphatase 2C alpha; PP2C alpha; Pp2c1; PP2CA; PP2Calpha; PP2C-ALPHA; PPM1A; PPPM1A; protein phosphatase 1A (formerly 2C), magnesium-dependent, alpha isoform; protein phosphatase 1A; protein phosphatase 2C alpha isoform; Protein phosphatase 2C isoform alpha; Protein phosphatase IA; protein phosphatase, Mg2+/Mn2+ dependent, 1A

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Citation for Human/Mouse/Rat PP2C alpha /PPM1A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Activation of PI3K/Akt Signaling Pathway in Rat Hypothalamus Induced by an Acute Oral Administration of D-Pinitol
    Authors: D Medina-Ver, JA Navarro, R Tovar, C Rosell-Val, A Gutiérrez-, JC Ledesma, C Sanjuan, FJ Pavón, E Baixeras, F Rodríguez, J Decara
    Nutrients, 2021-06-30;13(7):.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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