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Human/Mouse/Rat PP2C beta /PPM1B Antibody

Catalog #: AF4396
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4396
AF4396-SP
Detection of Human/Mouse/Rat PP2C beta /PPM1B by Western Blot.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat PP2C beta /PPM1B Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects endogenous human, mouse and rat PP2C beta in Western blots. In Western blots, this antibody does not cross‑react with recombinant human PP2C alpha or PP2Cδ.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human PP2C beta
Met1-Gly378
Accession # O75688
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/Rat PP2C beta /PPM1B antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat PP2C beta /PPM1B by Western Blot. Western blot shows lysates of CHP-100 human neuroblastoma cell line and A20 mouse B cell lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PP2C beta /PPM1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4396) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for PP2C beta/PPM1B at approximately 53 and 36 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PP2C beta/PPM1B

Protein phosphatase 2C beta (PP2C beta ), also called protein phosphatase, magnesium-dependent type 1B (PPM1B), preferentially dephosphorylates serine and threonine residues on proteins. There are five splice variants with sizes of 53, 43, 42, 36, and 21 kDa. This enzyme is resistant to inhibitors of other protein phosphatases such as okadaic acid and calyculin. Association of PP2C beta with I kappa B kinase inhibits its phosphorylating activity and is regulated by TNFalpha stimulation. PP2C beta is found in many cell types and tissues, especially muscle. The mRNA for PP2C beta accumulates in mature spermatocytes and metaphase-arrested, unfertilized oocytes. Knockout of PP2C beta in mice is lethal prior to embryo implantation.

Long Name
Protein Phosphatase 2C beta
Entrez Gene IDs
5495 (Human); 19043 (Mouse); 24667 (Rat)
Alternate Names
EC 3.1.3.16; MGC21657; PP2C beta; Pp2c2; PP2CB; PP2C-beta; PP2C-beta-X; PP2CBmagnesium-dependent, beta isoform; PPM1B; protein phosphatase 1B; Protein phosphatase 2C isoform beta; protein phosphatase 2C-like protein; protein phosphatase, Mg2+/Mn2+ dependent, 1B

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Citations for Human/Mouse/Rat PP2C beta /PPM1B Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. A heterozygous deficiency in protein phosphatase Ppm1b results in an altered ovulation number in mice
    Authors: N Ishii, T Homma, R Watanabe, N Kimura, M Ohnishi, T Kobayashi, J Fujii
    Mol Med Rep, 2019-04-25;0(0):.
  2. Protein phosphatase 5 modulates SMAD3 function in the transforming growth factor-beta pathway.
    Authors: Bruce D, Macartney T, Yong W, Shou W, Sapkota G
    Cell Signal, 2012-07-07;24(11):1999-2006.
    Species: Human, Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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