Human/Mouse/Rat Protocadherin-17 Antibody

Catalog #: AF7669 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7669
AF7669-SP

Detection of Mouse and Rat Protocadherin-17 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Protocadherin-17 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse and rat Protocadherin-17 in Western blots. In direct ELISAs, less than 5% cross-reactivity with Protocadherin-10 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Human embryonic kidney cell line HEK293-derived recombinant human Protocadherin-17
Leu18-Ser705
Accession # O14917

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse and Rat Protocadherin-17 by Western Blot. Western blot shows lysates of mouse brain (cortex) tissue and rat brain (cerebellum) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Protocadherin-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7669) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for Protocadherin-17 at approximately 120-160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Protocadherin-17

Protocadherin 17 (PCDH-17) is a 120-150 kDa glycoprotein, δ2-group member of the of the protocadherin subfamily, cadherin family of molecules. It is expressed on endothelium and epithelium such as stratified squamous epithelium and (likely) gastric columnar epithelium. PCDH17 is posited to act as an adhesion molecule, and appears to play a role in promoting cell cycle arrest and inhibiting migration. Mature human PCDH-17 is a type I transmembrane protein that is 1142 amino acids (aa) in length. It contains a 690 aa extracellular domain (ECD) (aa 18-707) plus a 431 aa cytoplasmic region. There are 6 cadherin domains in the ECD (aa 18-695). Two splice forms are reported that involve substitutions in the cytoplasmic region. The first possesses a 14 aa substitution, while the second shows a 25 aa substitution, both for aa 876-1159. Over aa 18-705, human PCDH-17 shares 98% aa identity with mouse PCDH-17.

Entrez Gene IDs

27253 (Human); 219228 (Mouse); 306055 (Rat)

Alternate Names

PCDH17; PCDH68; PCDH68protocadherin-68; PCH68; PCH68Protocadherin-68; protocadherin 17; protocadherin 68; Protocadherin17; Protocadherin-17