Human/Mouse/Rat Rab27a Antibody Summary
Ser135-Ala218
Accession # P51159
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human, Mouse, and Rat RAB27A by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, 786-O human renal cell adenocarcinoma cell line, MCF-7 human breast cancer cell line, Jurkat human acute T cell leukemia cell line, Rat-2 rat embryonic fibroblast cell line, and Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse/Rat RAB27A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7245) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for RAB27A at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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RAB27A in U937 Human Cell Line. RAB27A was detected in immersion fixed U937 human histiocytic lymphoma cell line using Sheep Anti-Human/Mouse/Rat RAB27A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7245) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
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Detection of Human Rab27a by Simple WesternTM. Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Rab27a at approximately 32 kDa (as indicated) using 5 µg/mL of Sheep Anti-Human/Mouse/Rat Rab27a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7245) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Detection of Rab27a by Immunoprecipitation. HAP1 near-haploid human cell line lysates were prepared and immunoprecipitation was performed using 2 ug of Sheep Anti-Human/Mouse/Rat Rab27a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7245) pre-coupled to Dynabeads Protein G. Immunoprecipitated Rab27a was detected in Western Blot with a Rabbit Rab27a antibody. The Ponceau stained transfer of the blot is shown. SM=4% starting material; UB=4% unbound fraction; IP=immunoprecipitate; HC=antibody heavy chain. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Rab27a
RAB27A (Ras-related protein Rab 27A; also GTP-binding protein Ram) is a 27-28 kDa member of the Rab27 subfamily, Rab family, Small GTPase superfamily of proteins. It is widely expressed, and found in cells diverse as mast cells, cytotoxic T cells, melanocytes, retinal pigment epithelium and pancreatic beta -cells. RAB27A plays a key role in the secretion of specialized lysosomes termed secretory lysosomes. In melanocytes, for example, RAB27A is incorporated into the melanosome membrane where it serves as a docking factor for melanophilin and myosin-Va, regulating melanosome transport to, and concentration at, sites of release. Human RAB27A is 221 amino acids (aa) in length. It contains multiple Rab family and subfamily motifs, and concludes with a C-terminal CXC prenylation sequence (aa 219‑221). There is one potential splice variant that shows a deletion of aa 146-153. Over aa 135-218, human RAB27A shares 92% and 94% aa sequence identity with mouse Rab27A and rat RAB27A, respectively.
Product Datasheets
Citations for Human/Mouse/Rat Rab27a Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Different Ability of Multidrug-Resistant and -Sensitive Counterpart Cells to Release and Capture Extracellular Vesicles
Authors: D Sousa, RT Lima, V Lopes-Rodr, E Gonzalez, F Royo, CPR Xavier, JM Falcón-Pér, MH Vasconcelo
Cells, 2021-10-26;10(11):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Tumor Extracellular Vesicles Regulate Macrophage-Driven Metastasis through CCL5
Authors: DC Rabe, ND Walker, FD Rustandy, J Wallace, J Lee, SL Stott, MR Rosner
Cancers, 2021-07-10;13(14):.
Species: Mouse
Sample Types: Whole Cells, Whole Tissue
Applications: Flow Cytometry, IHC
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