Human/Mouse/Rat Rap2A/B Antibody

Catalog #: MAB4698 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4698
MAB4698-SP

Detection of Human, Mouse, and Rat Rap2A/B by Western Blot.

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All Rap2A/B Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Rap2A/B Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat Rap2A/B in Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 455410

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Rap2A/B
Met1-Gln183
Accession # P10114

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat Rap2A/B by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat Rap2A/B Monoclonal Antibody (Catalog # MAB4698) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human Rap2A and Rap2B (5 ng/lane) were included. A specific band for Rap2A/B was detected at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Rap2A/B

Rap2A and Rap2B are members of the small GTPase superfamily and ras family of proteins. Both proteins are 183 amino acids (aa) in length (SwissProt P10114 and P61225), and they are 90% identical at the amino acid level, with the most variability at the carboxyl terminus of the protein. Unlike normal Ras proteins that have a nontransforming glutamine at aa 61, these two oncogenic proteins have a threonine in that position. This change makes the intrinsic GTPase activity for these proteins lower, thereby allowing them to exist in the activated state for a longer period of time than normal Ras proteins.

Long Name

Ras-related Protein 2A/B

Alternate Names

KREV;K-REV;RAP2;Ras-related protein Rap-2a;RbBP-30; Rap2A/B