Human/Mouse/Rat Rheb Antibody

Catalog #: MAB3426
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3426
MAB3426-SP

Detection of Human/Mouse/Rat Rheb by Western Blot.

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All Rheb Antibodies

Product Details

Citations (3)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Rheb Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat Rheb.

Source

Monoclonal Mouse IgG_2B Clone # 344912

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Rheb
Met1-Met184
Accession # AAH66307

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunocytochemistry

3-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse/Rat Rheb by Western Blot. Western blot shows lysates of NRK rat normal kidney cell line, Ramos human Burkitt's lymphoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Rheb Monoclonal Antibody (Catalog # MAB3426) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for Rheb at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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Rheb in Ramos Human Cell Line. Rheb was detected in immersion fixed Ramos human Burkitt's lymphoma cell line using Mouse Anti-Human/Mouse/Rat Rheb Monoclonal Antibody (Catalog # MAB3426) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunocytochemistry

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Detection of Rheb in NIH‑3T3 mouse embryonic fibroblast cell line (Positive) & PC‑12 rat adrenal pheochromocytoma cell line (Negative). Rheb was detected in immersion fixed NIH‑3T3 mouse embryonic fibroblast cell line (Positive) & PC‑12 rat adrenal pheochromocytoma cell line (Negative) using Mouse Anti-Human/Mouse/Rat Rheb Monoclonal Antibody (Catalog # MAB3426) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to Cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Rheb

Ras homolog enriched in brain (Rheb) is a novel member of the Ras superfamily of GTP-binding proteins involved in cell growth and cell cycle progression. Expressed in a wide range of tissues, Rheb was initially identified from its induction in rat hippocampal granule cells by seizures and NMDA-dependent synaptic activity. A complex containing the tuberous sclerosis gene products TSC1 and TSC2 functions as a GTPase-activating protein (GAP) for Rheb.

Long Name

Ras Homolog Enriched In Brain

Entrez Gene IDs

6009 (Human); 19744 (Mouse); 26954 (Rat)

Alternate Names

GTP-binding protein Rheb; MGC111559; Ras homolog enriched in brainRHEB2Ras homolog enriched in brain 2; Rheb; Rheb2

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse/Rat Rheb Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Single-Cell Spatial MIST for Versatile, Scalable Detection of Protein Markers
Authors: Meah, A;Vedarethinam, V;Bronstein, R;Gujarati, N;Jain, T;Mallipattu, SK;Li, Y;Wang, J;
Biosensors
Species: Mouse
Sample Types: Complex Sample Type
Applications: IHC
A beta-Cyclodextrin-Based Nanoparticle with Very High Transfection Efficiency Unveils siRNA-Activated TLR3 Responses in Human Prostate Cancer Cells
Authors: C de la Torr, P Játiva, I Posadas, D Manzanares, JLJ Blanco, CO Mellet, JMG Fernández, V Ceña
Pharmaceutics, 2022-11-09;14(11):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Inactivation of Rheb by PRAK-mediated phosphorylation is essential for energy-depletion-induced suppression of mTORC1.
Authors: Zheng M, Wang YH, Wu XN, Wu SQ, Lu BJ, Dong MQ, Zhang H, Sun P, Lin SC, Guan KL, Han J
Nat. Cell Biol., 2011-02-20;13(3):263-72.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot