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Human/Mouse/Rat RKIP Antibody

Catalog #: AF4538 4 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4538
AF4538-SP
Detection of Human/Mouse/Rat RKIP by Western Blot.
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat RKIP Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects endogenous human, mouse, and rat RKIP in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human RKIP
Met1-Lys187
Accession # P30086
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/Rat RKIP antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat RKIP by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells and A172 human glioblastoma cell line. PVDF membrane was probed with 0.2 µg/mL of Human/Mouse/Rat RKIP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4538) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for RKIP at approximately 23 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RKIP

Raf kinase inhibitor protein (RKIP) is a member of the phosphatidylethanolamine-binding protein (PEBP) family, and modulates the activation of several signal transduction cascades. By binding the MAP3K Raf-1, unphosphorylated RKIP inhibits activation of the MAP2K MEK. PKC-phosphorylated RKIP dissociates from Raf‑1 and inhibits GRK2, resulting in sustained G-protein signaling. Expressed in many tissues, RKIP is implicated in the regulation of such physiological processes as membrane biosynthesis, spermatogenesis, neural development, and metastasis suppression.

Long Name
Raf kinase inhibitory protein
Entrez Gene IDs
5037 (Human); 23980 (Mouse); 29542 (Rat)
Alternate Names
HCNP; HCNPpp; hippocampal cholinergic neurostimulating peptide; Neuropolypeptide h3; PBP; PBPPEBP-1; PEBP1; PEBP-1; PEBPHCNPpp; phosphatidylethanolamine binding protein 1; phosphatidylethanolamine-binding protein 1; prostatic binding protein; Prostatic-binding protein; Raf kinase inhibitory protein; RKIP; RKIPRaf kinase inhibitor protein

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Reviews for Human/Mouse/Rat RKIP Antibody

Average Rating: 3.8 (Based on 4 Reviews)

5 Star
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4 Star
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3 Star
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Human/Mouse/Rat RKIP Antibody
By Anonymous on 01/14/2021
Application: Microarrays Sample Tested: EDTA Plasma Species: Human
Other Human/Mouse/Rat RKIP Antibody AF4538
Human/Mouse/Rat RKIP Antibody
By Anonymous on 11/14/2018
Application: Microarrays Sample Tested: EDTA Plasma Species: Human
Human/Mouse/Rat RKIP Antibody
By Anonymous on 11/10/2018
Application: ELISA Sample Tested: Plasma Species: Human
Human/Mouse/Rat RKIP Antibody
By Anonymous on 11/02/2018
Application: MiAr Sample Tested: EDTA Plasma Species: Human