Human/Mouse/Rat SEC13 Antibody

Catalog #: MAB90552 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB90552-100
MAB90552-SP

Detection of Human, Mouse, and Rat SEC13 by Western Blot.

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All SEC13 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat SEC13 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human SEC13 in direct ELISAs and Western blots.

Source

Recombinant Monoclonal Rabbit IgG Clone # 1280C

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human SEC13
Gly123-Gln322
Accession # P55735

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat SEC13 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, C2C12 mouse myoblast cell line, NIH-3T3 mouse embryonic fibroblast cell line, and H4-II-E-C3 rat hepatoma cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/ Mouse/Rat SEC13 Monoclonal Antibody (Catalog # MAB90552) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for SEC13 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SEC13

Sec13 is a ubiquitously expressed protein which participates in the formation of vesicles in the COPII complex with Sec23p^.Sec24p, Sar1p and Sec31. The COPII vesicle budding complex was first described in the yeast system and was shown to provide coat proteins in the early secretory pathway. Sec13 is recruited to endoplasmic reticulum (ER) membranes where it forms a three dimensional cuboctahedron cage lattice structure by association with Sec31. This vesicular structure is necessary for ER-Golgi transport. Presenillin-1, which is implicated in early-onset Alzheimer's disease has been shown to interact with Sec13 vesicles. Additionally, Sec13 has been shown to be a component of the Y-shaped Nup107-160 subcomplex of the nuclear pore complex (NPC), and the GATOR2 sub-complex of the GATOR complex.

Long Name

Nuclear Pore Complex Protein Nup85

Entrez Gene IDs

6396 (Human); 110379 (Mouse); 297522 (Rat)

Alternate Names

D3S1231E; D3S1231ESEC13L1npp-20; Npp-20; protein SEC13 homolog; SEC13 (S. cerevisiae)-like 1; SEC13 homolog (S. cerevisiae); SEC13; SEC13L1; SEC13-like 1 isoform; SEC13-like protein 1; SEC13R; SEC13-related protein; SEC13RSEC13-like 1 (S. cerevisiae)