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Human/Mouse/Rat Secretagogin Antibody

Catalog #: AF4878
7 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4878
AF4878-SP
Detection of Human/Mouse/Rat Secretagogin by Western Blot.
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Citations (7)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Secretagogin Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects mouse Secretagogin in Western blots. Reactivity with human, rat, and other species is expected due to the high sequence homology of the immunogen used.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Secretagogin
Asn164-Pro276
Accession # O76038
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/Rat Secretagogin antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat Secretagogin by Western Blot. Western blot shows lysates of beta TC-6 mouse beta cell insulinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Secretagogin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4878) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Secretagogin at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human and Mouse Secretagogin antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human and Mouse Secretagogin by Simple WesternTM. Simple Western lane view shows lysates of beta TC-6 mouse beta cell insulinoma cell line and human pancreas tissue, loaded at 0.2 mg/mL. A specific band was detected for Secretagogin at approximately 38 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat Secretagogin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4878) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Secretagogin

Secretagogin (SCGN) is a 32 kDa member of the EF-hand family of Ca-binding proteins. It is expressed in select neurons, as well as neuroendocrine cells and pancreatic islets. Human Secretagogin is 276 amino acids (aa) in length and contains six 35 aa EF-hand domains (aa 12‑276). There is one alternative splice variant that shows a 22 aa substitution for aa 28‑276, and which demonstrates no Ca-binding ability. Secretagogin is known to circulate, presumably released during cell apoptosis. Over aa 164‑276, human Secretagogin is more than 97% aa identical to mouse, porcine and canine Secretagogin.

Entrez Gene IDs
10590 (Human); 214189 (Mouse); 306942 (Rat)
Alternate Names
CALBL; DJ501N12.8; SCGN; SECRET; Secretagogin; secretagogin, EF-hand calcium binding protein; SECRETsecretagogin; SEGN; Setagin

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Citations for Human/Mouse/Rat Secretagogin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Secretagogin expression delineates functionally-specialized populations of striatal parvalbumin-containing interneurons
    Authors: Farid N Garas, Rahul S Shah, Eszter Kormann, Natalie M Doig, Federica Vinciati, Kouichi C Nakamura et al.
    eLife
  2. Organizational motifs for ground squirrel cone bipolar cells
    Authors: Adam C. Light, Yongling Zhu, Jun Shi, Shannon Saszik, Sarah Lindstrom, Laura Davidson et al.
    The Journal of Comparative Neurology
  3. Structural and molecular heterogeneity of calretinin-expressing interneurons in the rodent and primate striatum
    Authors: Farid N. Garas, Eszter Kormann, Rahul S. Shah, Federica Vinciati, Yoland Smith, Peter J. Magill et al.
    Journal of Comparative Neurology
  4. GABAergic medial septal neurons with low-rhythmic firing innervating the dentate gyrus and hippocampal area CA3.
    Authors: Salib M, Joshi A, Katona L et al.
    J Neurosci.
  5. Secretagogin marks amygdaloid PKC&delta interneurons and modulates NMDA receptor availability
    Authors: Z Hevesi, D Zelena, RA Romanov, J Hanics, A Ignácz, A Zambon, DD Pollak, D Lendvai, K Schlett, M Palkovits, T Harkany, TGM Hökfelt, A Alpár
    Proceedings of the National Academy of Sciences of the United States of America, 2021-02-16;118(7):.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Immunoprecipitation
  6. Secretagogin is a Ca2+-binding protein identifying prospective extended amygdala neurons in the developing mammalian telencephalon.
    Authors: Mulder J, Spence L, Tortoriello G, Dinieri JA, Uhlen M, Shui B, Kotlikoff MI, Yanagawa Y, Aujard F, Hokfelt T, Hurd YL, Harkany T
    Eur. J. Neurosci., 2010-06-07;31(12):2166-77.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC-Fr, Western Blot
  7. Hypothalamic CNTF volume transmission shapes cortical noradrenergic excitability upon acute stress
    Authors: Alán Alpár, Péter Zahola, János Hanics, Zsófia Hevesi, Solomiia Korchynska, Marco Benevento et al.
    The EMBO Journal

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