Human/Mouse/Rat SHP-1 Antibody Summary
Ala205-Lys595
Accession # P29350
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![Detection of Human/Mouse/Rat SHP-1 antibody by Western Blot. Detection of Human/Mouse/Rat SHP-1 antibody by Western Blot.](https://resources.rndsystems.com/images/datasheets/antibody/SHP-1_AF1878_Western_Blot_5151.jpg)
Detection of Human/Mouse/Rat SHP‑1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human/Mouse/Rat SHP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1878) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, Recombinant Human Active SHP-1 aa 205-595 (Catalog # 1878-SH) and Recombinant Human SHP-2 (Catalog # 1894-SH) (2 ng/lane) were included. A specific band for SHP-1 was detected at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
![Detection of Human SHP-1 antibody by Simple Western<SUP>TM</SUP>. Detection of Human SHP-1 antibody by Simple Western<SUP>TM</SUP>.](https://resources.rndsystems.com/images/datasheets/antibody/SHP1_AF1878_Simple_Western_16681.jpg)
Detection of Human SHP‑1 by Simple WesternTM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for SHP-1 at approximately 65 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat SHP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1878) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SHP-1
Src-Homology 2 domain Phosphatase-1 (SHP-1), also known as Protein Tyrosine Phosphatase 1C (PTP1C), PTPN6, and Hematopoetic Cell Phosphatase (HCPH), is an enzyme that selectively dephosphorylates tyrosine residues in proteins. Spontaneous point mutations in the SHP-1 gene in mice produce the “motheaten” and "motheaten viable” phenotypes that are severely autoimmune and immunodeficient (1). The enzyme is highly expressed in leukocyte cell types (2). SHP-1 has a regulatory region containing two Src homology 2 (SH2) domains that are critical for its binding to ITIM domains in inhibitory immunoreceptors (3). Deletion of the SH2 domains, as in this product, causes a marked increase in phosphatase activity (4). SHP-1 will dephosphorylate a wide variety of proteins, including the EGF receptor (5). A phosphopeptide containing the EGFR (Y992) sequence (R&D Systems, Catalog # ES006) can be used to measure the activity of SHP-1 by detecting the release of phosphate (R&D Systems, Catalog # DY996).
- Tsui, H.W. et al. (1993) Nature Genet. 4:124.
- Matthews, R.J. et al. (1992) Mol. Cell. Biol. 12:2396.
- Burshtyn, D.N. et al. (1997) J. Biol. Chem. 272:13066.
- Pei, D. et al. (1994) Biochemistry 33:15483.
- Tomic, S. et al. (1995) J. Biol. Chem. 270:21277.
Product Datasheets
Citation for Human/Mouse/Rat SHP-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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S100A8 induces IL-10 and protects against acute lung injury.
Authors: Hiroshima Y, Hsu K, Tedla N, Chung Y, Chow S, Herbert C, Geczy C
J Immunol, 2014-02-14;192(6):2800-11.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
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