Human/Mouse/Rat SIRP delta Antibody

Catalog #: MAB101381 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB101381-100
MAB101381-SP

Detection of Human, Mouse, and Rat SIRP delta by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat SIRP delta Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human SIRP delta in direct ELISAs. Detects human, mouse, and rat SIRP delta in Western blots.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2588B

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Human embryonic kidney cell HEK293-derived human SIRP delta
Phe30-Arg197
Accession # Q9H106

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

Human testis tissue, Mouse testis tissue, and Rat testis tissue

Immunocytochemistry

3-25 µg/mL

Immersion fixed THP‑1 human acute monocytic leukemia cell line and immersion fixed U937 human histiocytic lymphoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat SIRP delta by Western Blot. Western blot shows lysates of human testis tissue, mouse testis tissue, and rat testis tissue. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human/Mouse/Rat SIRP delta Monoclonal Antibody (Catalog # MAB101381) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody HAF008. A specific band was detected for SIRP delta at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using our Western Blot Buffer Group 1.

Immunocytochemistry

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SIRP delta in THP‑1 Human Cell Line. SIRP delta was detected in immersion fixed THP‑1 human acute monocytic leukemia cell line (left panel; positive staining) and MCF‑7 human breast cancer cell line (right panel; negative staining) using Rabbit Anti-Human/Mouse/Rat SIRP delta Monoclonal Antibody (Catalog # MAB101381) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunocytochemistry

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SIRP delta in U937 Human Cell Line. SIRP delta was detected in immersion fixed U937 human histiocytic lymphoma cell line (left panel; positive staining) and MCF‑7 human breast cancer cell line (right panel; negative staining) using Rabbit Anti-Human/Mouse/Rat SIRP delta Monoclonal Antibody (Catalog # MAB101381) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol forFluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SIRP delta

SIRP delta (SIRPD; Signal Regulatory Protein Delta), also know as Protein Tyrosine Phosphatase non-Receptor Type Substrate 1-l Like 2 (PTPNS1L2), is member of the signal regulatory proteins (SIRPS) family (1). SIRPD contains a 168 amino acid Ig-like domain that is characteristic of other SIRP family members (1). Unlike other members of SIRPS family, SIRPD lacks the transmemberane region, and is secreted (2). Murine homologs of SIRPD are not characterized. Expression sequence tag analysis suggests that SIRPD may be expressed in sperm cells and respiratory tissue (2). Using BioPlex 2.0 (Biophysical Interactions of ORFeome-derived complexes) high-throughput affinity purification–mass spectrometry (AP–MS) analysis to identify probable protein–protein interactions, several candidate SIRPD interactions were found including DIRAS2 (3). In-house testing indicates SIRPD can interact with DIRAS2.

References

Van den Berg, T.K. et al. (2005) J. Immunol. 175:7788.
Van Beek, E.M. et al. (2005) J Immunol. 175:7781.
Huttlin, E.L. et al. (2017) Nature 545:505.

Long Name

Signal-regulatory Protein delta

Entrez Gene IDs

128646 (Human)

Alternate Names

dJ576H24.4; Protein tyrosine phosphatase non-receptor type substrate 1-like 2; protein tyrosine phosphatase, non-receptor type substrate 1-like 2; PTPNS1L2; signal-regulatory protein delta; SIRP delta; SIRPD; SIRP-Delta