Human/Mouse/Rat TDP-43/TARDBP Antibody

Detection of Human TDP-43/TARDBP by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 0.4 µg/mL of Mouse Anti-Human/Mouse/Rat TDP-43/TARDBP Monoclonal Antibody (Catalog # MAB7778) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for TDP-43/TARDBP at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human and Mouse TDP-43/TARDBP by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human TDP-43/TARDBP Monoclonal Antibody (Catalog # MAB7778) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for TDP-43/TARDBP at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

TDP-43/TARDBP in A431 Human Cell Line. TDP-43/TARDBP was detected in immersion fixed A431 human epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat TDP-43/TARDBP Monoclonal Antibody (Catalog # MAB7778) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

TDP-43/TARDBP in Human Brain. TDP-43/TARDBP was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Mouse Anti-Human/Mouse/Rat TDP-43/TARDBP Monoclonal Antibody (Catalog # MAB7778) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human and Rat TDP-43/TARDBP by Simple Western^TM. Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line and NR8383 rat alveolar macrophage cell line, loaded at 0.2 mg/mL. A specific band was detected for TDP-43/TARDBP at approximately 54 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human TDP-43/TARDBP Monoclonal Antibody (Catalog # MAB7778). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Detection of TDP-43/TARDBP by Immunoprecipitation. Immunoprecipitation was performed on cell lysate of HAP1 human near-haploid cell line using 2.0 μg of Mouse Anti-Human TDP-43 Monoclonal Antibody (Catalog # MAB7778) pre-coupled to protein G or protein A beads. Immunoprecipitated TDP-43/TARDBP was detected with a Rabbit Anti-TDP-43 antibody. The Ponceau stained transfers of each blot are shown. SM=10% starting material; UB=10% unbound fraction; IP=immunoprecipitated. Image, protocol, and testing courtesy of YCharOS Inc. (ycharos.com).

Detection of Mouse TDP-43/TARDBP by Western Blot FTLD‐related pathological changes in Tmem106b−/−Grn−/− miceA, BWestern blot analysis of p62, ubiquitin (Ub), TDP‐43 and p‐TDP-43 in RIPA‐ and urea‐soluble fractions from spinal cord (C5–C8) and brain of 5‐month‐old WT, Tmem106b−/−, Grn−/−, and Tmem106b−/−Grn−/− mice. Spinal cord: n = 3; brain: n = 4. Data presented as mean ± SEM. One‐way ANOVA tests with Bonferroni's multiple comparisons: *P < 0.05; **P < 0.01, ***P < 0.001, ****P < 0.0001.C, DImmunostaining of p62 and ubiquitin in the spinal cord sections from 5‐month‐old WT, Tmem106b−/−, Grn−/−, and Tmem106b−/−Grn−/− mice. The number of p62 or Ub positive puncta was quantified. n = 3. Data presented as mean ± SEM. One‐way ANOVA tests with Bonferroni's multiple comparisons: ***P < 0.001, ****P < 0.0001. Scale bar = 10 μm.Source data are available online for this figure. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32852886), licensed under a CC-BY license. Not internally tested by R&D Systems.