Human/Mouse/Rat TRAF-3 Isoform 2 Antibody

Catalog #: MAB3278 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3278
MAB3278-SP

Detection of Human/Mouse/Rat TRAF‑3 Isoform 2 by Western Blot.

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Human/Mouse/Rat TRAF-3 Isoform 2 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects endogenous human, mouse, and rat TRAF-3 in Western blots. In Western blots, this antibody does not cross-react with recombinant human TRAF-1, -2, -4, -5, or -6.

Source

Monoclonal Rat IgG_2A Clone # 433113

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human TRAF-3
Met1-Pro543
Accession # NP_663778

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse/Rat TRAF‑3 Isoform 2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, A20 mouse B cell lymphoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Human/Mouse/Rat TRAF-3 Isoform 2 Monoclonal Antibody (Catalog # MAB3278) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for TRAF-3 Isoform 2 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRAF-3

Tumor Necrosis Factor (TNF) Receptor-Associated Factors (TRAFs) are a family of adaptor proteins that interact with a wide range of cell surface receptors and participate in the regulation of cell survival, proliferation, differentiation, and stress response. TRAFs were identified by their ability to form complexes with TNF receptor superfamily members but more recently are reported to also bind to Toll/IL-1 receptor family members and mediate cellular signaling. Seven members of the TRAF family have been identified. All TRAF proteins have a homologous C-terminal TRAF domain that can bind the cytoplasmic domain of receptors as well as other TRAFs. TRAFs 2‑6 have N-terminal RING and zinc finger domains that are involved in signaling downstream events. Two isoforms of TRAF-3 have been identified, a 568 amino acid, 64 kDa protein (isoform 1, also known as CD40BP and CRAF1) and a 543 amino acid, 62 kDa protein (isoform 2, also known as CAP1 and LAP1). TRAF-3 has been shown bind specifically to the PEQET sequence motif on the cytoplasmic domain of CD40. TRAF-3 and TRAF-6 have also been identified as being involved in TLR signaling events.

Long Name

TNF Receptor-Associated Factor 3

Entrez Gene IDs

7187 (Human); 22031 (Mouse)

Alternate Names

CAP1; CAP-1CD40 receptor associated factor 1; CD40 associated protein 1; CD40 binding protein; CD40 receptor-associated factor 1; CD40-binding protein; CD40bp; CRAF1; CRAF1CAP1; LAP1; LAP1CD40BP; LMP1-associated protein 1; TNF receptor-associated factor 3; TRAF3; TRAF-3