Human/Mouse/Rat TRRAP Antibody

Catalog #: AF7365 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7365
AF7365-SP

Detection of Human, Mouse, and Rat TRRAP by Western Blot.

1 Image

All TRRAP Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat TRRAP Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse and rat TRRAP in Western blots. Detects recombinant human TRRAP in direct ELISAs.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human TRRAP
Asn3610-Leu3859, predicted
Accession # Q9Y4A5

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human, Mouse, and Rat TRRAP by Western Blot. Western blot shows lysates of MOLT-4 human acute lymphoblastic leukemia cell line, HeLa human cervical epithelial carcinoma cell line, mouse placenta tissue, mouse uterus tissue, and rat ovary tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human TRRAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7365) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TRRAP at approximately 440 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRRAP

TRRAP (Transformation/transcription domain-associated protein; also PAF350/400 and STAF40) is a 400-450 kDa member of the TRA1 subfamily, PI3/PI4 kinase family, ATM superfamily of molecules. It is a ubiquitously expressed nuclear protein that functions as a promoter of gene transcription. In particular, the activity of the E2F transcription factor family is regulated by the degree of histone acetylation. Deacetylation inhibits E2F-mediated transcription, while acetylation promotes gene transcription. TRRAP promotes histone acetylation by serving as a positive modulator/cofactor for GCN5 acetyltransferase activity. TRRAP also acts independently of GCN5. It serves as a recruiter for Skp1, a ubiquitin ligase that ubiquitates chromatin-bound beta -catenin, leading to a downregulation of Wnt-signaling activity. Human TRRAP is 3859 amino acids (aa) in length. It contains one Pro-rich region (aa 485-526), an NLS (aa 2047-2062), two FAT domains (aa 2704-3275 and 3827-3859) and a nonenzymatic PI3/PI4 kinase domain (aa 3528-3826). There are multiple potential isoform variants. They show various combinations of a deletion involving aa 1493-1510, an Ala substitution for aa 3001-3012, a 119 aa substitution for aa 3784-3859, and an 18 aa insertion after Asp3287. Over aa 3610-3859, human TRRAP shares 99% aa identity with mouse TRRAP.

Long Name

Transformation/Transcription Domain-associated Protein

Entrez Gene IDs

8295 (Human)

Alternate Names

350/400 kDa PCAF-associated factor; FLJ10671; PAF350/400; PAF400; PAF400STAF40; STAF40; Tra1 homolog; Tra1; transformation/transcription domain-associated protein; TR-AP; TRRAP