Human/Mouse/Rat UBASH3B/STS1 Antibody

Catalog #: AF6696 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6696
AF6696-SP

Detection of Human and Rat UBASH3B/STS1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat UBASH3B/STS1 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human and rat UBASH3B/STS1 in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human UBASH3B/STS1
Gly48-Phe253
Accession # Q8TF42

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Rat UBASH3B/STS1 by Western Blot. Western blot shows lysates of HCT-116 human colorectal carcinoma cell line, HEK293 human embryonic kidney cell line, MOLT-4 human acute lymphoblastic leukemia cell line, U-118-MG human glioblastoma/astrocytoma cell line, U2OS human osteosarcoma cell line, and Y3-Ag rat myeloid cell line. PVDF Membrane was probed with 2 µg/mL of Human/Rat UBASH3B/STS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6696) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for UBASH3B/STS1 at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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UBASH3B/STS1 in HeLa Human Cell Line. UBASH3B/STS1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human/Mouse/Rat UBASH3B/STS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6696) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: UBASH3B/STS1

UBASH3B (Ubiquitin-associated domain SH3 domain-containing protein B; also STS1 and TULA2) is a cytosolic 72-74 kDa member of the TULA (T cell ubiquitin ligand) family of proteins. It is widely expressed and appears to perform multiple functions. As a histidine phosphatase, it dephosphorylates Syk and Syk-related molecules, thus dampening signaling pathways. Via ubiquitin and SH3 binding domains, it binds to monoubiquitinated RTKs and Cbl, thereby increasing RTK activity by blocking its polyubiquitination and subsequent lysosomal degradation. There is also the potential for phosphodiesterase activity directed towards tRNA activity. Human UBASH3B is 649 amino acids (aa) in length. It contains one UBA domain (aa 39-75), a proline-rich ligand binding SH3 region (aa 257-316), and a PGM/histidine phosphatase domain (aa 386-598). UBASH3B is suggested to dimerize. Over aa 47-253, human and mouse UBASH3B are identical in amino acid sequence.

Long Name

Ubiquitin Associated and SH3 Domain Containing Protein B

Entrez Gene IDs

84959 (Human); 72828 (Mouse); 315579 (Rat)

Alternate Names

Cbl-interacting Protein p70; Cbl-interacting protein Sts-1; KIAA1959SH3 domain-containing 70 kDa protein, suppressor of T-cell receptor signaling1, nm23-phosphorylated unknown substrate; MGC15437; nm23-phosphorylated unknown substrate; p70; STS1; STS1EC 3.1.3.48; STS-1ubiquitin-associated and SH3 domain-containing protein B; Suppressor of T-cell receptor signaling 1; T-cell ubiquitin ligand 2; TULA-2; Tyrosine-protein phosphatase STS1/TULA2; UBASH3B; ubiquitin associated and SH3 domain containing B